Distinct short-range ovule signals attract or repel arabidopsis thaliana pollen tubes in vitro

Ravishankar Palanivelu, Daphne Preuss

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Background: Pollen tubes deliver sperm after navigating through flower tissues in response to attractive and repulsive cues. Genetic analyses in maize and Arabidopsis thaliana and cell ablation studies in Torenia fournieri have shown that the female gametophyte (the 7-celled haploid embryo sac within an ovule) and surrounding diploid tissues are essential for guiding pollen tubes to ovules. The variety and inaccessibility of these cells and tissues has made it challenging to characterize the sources of guidance signals and the dynamic responses they elicit in the pollen tubes.Results: Here we developed an in vitro assay to study pollen tube guidance to excised A. thaliana ovules. Using this assay we discerned the temporal and spatial regulation and species-specificity of late stage guidance signals and characterized the dynamics of pollen tube responses. We established that unfertilized A. thaliana ovules emit diffusible, developmentally regulated, species-specific attractants, and demonstrated that ovules penetrated by pollen tubes rapidly release diffusible pollen tube repellents. Conclusion: These results demonstrate that in vitro pollen tube guidance to excised A. thaliana ovules efficiently recapitulates much of in vivo pollen tube behaviour during the final stages of pollen tube growth. This assay will aid in confirming the roles of candidate guidance molecules, exploring the phenotypes of A. thaliana pollen tube guidance mutants and characterizing interspecies pollination interactions.

Original languageEnglish (US)
Title of host publicationReproductive Physiology in Plants
PublisherApple Academic Press
Pages301-319
Number of pages19
ISBN (Electronic)9781466561977
ISBN (Print)9781926692647
StatePublished - Jan 1 2011
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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  • Cite this

    Palanivelu, R., & Preuss, D. (2011). Distinct short-range ovule signals attract or repel arabidopsis thaliana pollen tubes in vitro. In Reproductive Physiology in Plants (pp. 301-319). Apple Academic Press.