Distribution and estrogen regulation of membrane progesterone receptor-β in the female rat brain

Damian G. Zuloaga, Stephanie L. Yahn, Yefei Pang, Alicia M. Quihuis, Mario G. Oyola, Andrea Reyna, Peter Thomas, Robert J Handa, Shailaja K. Mani

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Abstract

Although several studies have reported the localization of membrane progesterone (P 4) receptors (mPR) in various tissues, few have attempted to describe the distribution and regulation of these receptors in the brain. In the present study, we investigated expression of two mPR subtypes, mPRαand mPRβ, within regions of the brain, known to express estradiol (E 2)-dependent [preoptic area (POA) and hypothalamus] and independent (cortex) classical progestin receptors. Saturation binding and Scatchard analyses on plasma membranes prepared from rat cortex, hypothalamus, and POA demonstrated high-affinity, specific P 4-binding sites characteristic of mPR. Using quantitative RT-PCR, we found that mPRβ mRNA was expressed at higher levels than mPRα, indicating that mPRβ may be the primary mPR subtype in the rat brain. We also mapped the distribution of mPRβprotein using immunohistochemistry. The mPRβ-immunoreactive neurons were highly expressed in select nuclei of the hypothalamus (paraventricular nucleus, ventromedial hypothalamus, and arcuate nucleus), forebrain (medial septum and horizontal diagonal band), and midbrain (oculomotor and red nuclei) and throughout many areas of the cortex and thalamus. Treatment of ovariectomized female rats with E 2 benzoate increased mPRβ immunoreactivity within the medial septum but not the medial POA, horizontal diagonal band, or oculomotor nucleus. Together, these findings demonstrate a wide distribution of mPRβ in the rodent brain that may contribute to functions affecting behavioral, endocrine, motor, and sensory systems. Furthermore, E 2 regulation of mPRβ indicates a mechanism through which estrogens can regulate P 4 function within discrete brain regions to potentially impact behavior.

Original languageEnglish (US)
Pages (from-to)4432-4443
Number of pages12
JournalEndocrinology
Volume153
Issue number9
DOIs
StatePublished - Sep 1 2012

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Progesterone Receptors
Estrogens
Preoptic Area
Membranes
Hypothalamus
Brain
Red Nucleus
Septal Nuclei
Arcuate Nucleus of Hypothalamus
Paraventricular Hypothalamic Nucleus
Benzoates
Prosencephalon
Mesencephalon
Thalamus
Progesterone
Estradiol
Rodentia
Immunohistochemistry
Binding Sites
Cell Membrane

ASJC Scopus subject areas

  • Endocrinology

Cite this

Zuloaga, D. G., Yahn, S. L., Pang, Y., Quihuis, A. M., Oyola, M. G., Reyna, A., ... Mani, S. K. (2012). Distribution and estrogen regulation of membrane progesterone receptor-β in the female rat brain. Endocrinology, 153(9), 4432-4443. https://doi.org/10.1210/en.2012-1469

Distribution and estrogen regulation of membrane progesterone receptor-β in the female rat brain. / Zuloaga, Damian G.; Yahn, Stephanie L.; Pang, Yefei; Quihuis, Alicia M.; Oyola, Mario G.; Reyna, Andrea; Thomas, Peter; Handa, Robert J; Mani, Shailaja K.

In: Endocrinology, Vol. 153, No. 9, 01.09.2012, p. 4432-4443.

Research output: Contribution to journalArticle

Zuloaga, DG, Yahn, SL, Pang, Y, Quihuis, AM, Oyola, MG, Reyna, A, Thomas, P, Handa, RJ & Mani, SK 2012, 'Distribution and estrogen regulation of membrane progesterone receptor-β in the female rat brain', Endocrinology, vol. 153, no. 9, pp. 4432-4443. https://doi.org/10.1210/en.2012-1469
Zuloaga DG, Yahn SL, Pang Y, Quihuis AM, Oyola MG, Reyna A et al. Distribution and estrogen regulation of membrane progesterone receptor-β in the female rat brain. Endocrinology. 2012 Sep 1;153(9):4432-4443. https://doi.org/10.1210/en.2012-1469
Zuloaga, Damian G. ; Yahn, Stephanie L. ; Pang, Yefei ; Quihuis, Alicia M. ; Oyola, Mario G. ; Reyna, Andrea ; Thomas, Peter ; Handa, Robert J ; Mani, Shailaja K. / Distribution and estrogen regulation of membrane progesterone receptor-β in the female rat brain. In: Endocrinology. 2012 ; Vol. 153, No. 9. pp. 4432-4443.
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