Distribution of androgen receptor in microdissected brain areas of the female baboon (Papio cynocephalus)

Robert J Handa, Charles E. Roselli, John A. Resko

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We measured androgen receptors in the brain and pituitary of 4 female baboons (Papio cynocephalus) by the in vitro binding of methyltrienolone (R1881) to cytosols from 17 brain subregions as well as anterior and posterior pituitaries. High levels of AR were detected in anterior (22.1 ± 7.1 (S.E.M.) fmol/mg protein) and posterior pituitary (12.6 ± 3.3 fmol/mg protein). In brain tissue, the highest androgen receptor levels were found in the infundibular nucleus/median eminence (9.4 ± 2.3 fmol/mg protein), ventromedial nucleus (6.3 ± 1.7 fmol/mg protein) and periventricular area (4.9 ± 1.3 fmol/mg protein). Saturation analysis of anterior pituitary and brain tissue (pool of hypothalamic, preoptic area, amygdala and septum remaining after microdissection of brain nuclei) showed that [3H]R1881 binds to the androgen receptor with high specificity and affinity (Kd = 1.25 × 10-10 M, 0.45 × 10-10 M, in anterior pituitary and HPA cytosol, respectively). Serum testosterone levels were low in all animals (0.59 ± 0.26 ng/ml). With these data we described the quantitative distribution of androgen receptor in the pituitary and in specific brain nuclei in a species of nonhuman primate. The distribution is similar in many respects to that described in the male rat and the data suggest a conservation of androgen receptor distribution across species.

Original languageEnglish (US)
Pages (from-to)111-116
Number of pages6
JournalBrain Research
Volume445
Issue number1
DOIs
StatePublished - Mar 29 1988
Externally publishedYes

Fingerprint

Papio cynocephalus
Papio
Androgen Receptors
Metribolone
Brain
Proteins
Cytosol
Microdissection
Median Eminence
Arcuate Nucleus of Hypothalamus
Preoptic Area
Amygdala
Primates
Testosterone
Serum

Keywords

  • Androgen receptor
  • Baboon
  • R1881

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Distribution of androgen receptor in microdissected brain areas of the female baboon (Papio cynocephalus). / Handa, Robert J; Roselli, Charles E.; Resko, John A.

In: Brain Research, Vol. 445, No. 1, 29.03.1988, p. 111-116.

Research output: Contribution to journalArticle

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