Disturbances of amino acids from temporal lobe synaptosomes in human complex partial epilepsy

David M. Labiner, C. C. Yan, Martin E. Weinand, Ryan J. Huxtable

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We have studied the levels of neuroactive amino acids in synaptosomes (P2 fraction) isolated from brain tissue of ten patients with medically intractable epilepsy who were undergoing temporal lobectomy. First, lateral temporal tissue (nonfocal) was removed followed by medial temporal tissue (focal). A synaptosomal fraction (P2) was immediately prepared from each tissue and analyzed for free amino acid concentrations. Statistically significant reductions were seen in glutamine and GABA concentrations in focal tissue compared to nonfocal tissue. The ratio of excitatory amino acids (aspartate and glutamate) to inhibitory amino acids (taurine and GABA) was significantly higher in focal tissue compared to nonfocal. The glutamine/glutamate ratio was significantly reduced. These data support the hypothesis that alterations in the balance between excitatory and inhibitory amino acids may be involved in the expression of epilepsy.

Original languageEnglish (US)
Pages (from-to)1379-1383
Number of pages5
JournalNeurochemical Research
Volume24
Issue number11
DOIs
StatePublished - Nov 9 1999

Keywords

  • Amino acids
  • Epilepsy
  • GABA
  • Glutamate
  • Glutamine
  • Synaptosomes
  • Taurine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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