Diurnal Regulation of Plant Epidermal Wax Synthesis through Antagonistic Roles of the Transcription Factors SPL9 and DEWAX

Rong Jun Li, Lin Mao Li, Xiu Lin Liu, Jang Chol Kim, Matthew A. Jenks, Shiyou Lü

Research output: Contribution to journalArticle

Abstract

Plant surface waxes form an outer barrier that protects the plant from many forms of environmental stress. The deposition of cuticular waxes on the plant surface is regulated by external environmental changes, including light and dark cycles. However, the underlying molecular mechanisms controlling light regulation of wax production are still poorly understood, especially at the posttranscriptional level. In this paper, we report the regulation of cuticular wax production by the miR156-SPL9 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9) module in Arabidopsis (Arabidopsis thaliana). When compared with wild-type plants, miR156 and SPL9 mutants showed significantly altered cuticular wax amounts in both stems and leaves. Furthermore, it was found that SPL9 positively regulates gene expression of the alkane-forming enzyme ECERIFERUM1 (CER1), as well as the primary (1-) alcohol-forming enzyme ECERIFERUM4 (CER4), to enhance alkane and 1-alcohol synthesis, respectively. Our results indicate that complex formation of SPL9 with a negative regulator of wax synthesis, DEWAX, will hamper SPL9 DNA binding ability, possibly by interfering with SPL9 homodimerization. Combined with their diurnal gene and protein expressions, this dynamic repression-activation transcriptional module defines a dynamic mechanism that may allow plants to optimize wax synthesis during daily cycles. These findings provide a regulatory framework for environmental signal integration in the regulation of wax synthesis.

Original languageEnglish (US)
Pages (from-to)2711-2733
Number of pages23
JournalThe Plant cell
Volume31
Issue number11
DOIs
StatePublished - Nov 1 2019
Externally publishedYes

Fingerprint

Waxes
waxes
Transcription Factors
transcription factors
epicuticular wax
synthesis
alkanes
alcohols
Alkanes
gene expression
Arabidopsis
binding capacity
transcriptional activation
enzymes
Alcohols
binding proteins
Gene Expression
Arabidopsis thaliana
protein synthesis
promoter regions

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Diurnal Regulation of Plant Epidermal Wax Synthesis through Antagonistic Roles of the Transcription Factors SPL9 and DEWAX. / Li, Rong Jun; Li, Lin Mao; Liu, Xiu Lin; Kim, Jang Chol; Jenks, Matthew A.; Lü, Shiyou.

In: The Plant cell, Vol. 31, No. 11, 01.11.2019, p. 2711-2733.

Research output: Contribution to journalArticle

Li, Rong Jun ; Li, Lin Mao ; Liu, Xiu Lin ; Kim, Jang Chol ; Jenks, Matthew A. ; Lü, Shiyou. / Diurnal Regulation of Plant Epidermal Wax Synthesis through Antagonistic Roles of the Transcription Factors SPL9 and DEWAX. In: The Plant cell. 2019 ; Vol. 31, No. 11. pp. 2711-2733.
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