Divergent effects of cocaine on cytokine production by lymphocytes and monocyte/macrophages

HIV-1 enhancement by cocaine within the blood-brain barrier

A. M. Fiala, X. H. Gan, T. Newton, F. Chiappelli, P. Shapshak, V. Kermani, M. A. Kung, A. Diagne, O. Martinez, D. Way, Martin E Weinand, Marlys H Witte, M. Graves

Research output: Contribution to journalArticle

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Abstract

Cocaine-related immunosuppression is assumed to have serious consequences, but its evaluation in drug-addicted subjects is lacking. In this study performed with materials from addicted subjects receiving intravenous cocaine and normal control subjects, acute cocaine effects on cytokine production in vivo and in mononuclear cells in vitro were determined. Acute intravenous cocaine administration resulted in (a) increased white blood cell and lymphocyte courts, (b) decreased tumor necrosis factor-α (TNF-α) and interleukin (IL)-10 serum levels; (c) depressed TNF-α, IL-10 and IL-12 production by unstimulated or LPS- stimulated mononuclear cells; (d) increased TNF-α production by PHA- stimulated mononuclear cells. These observations suggest that cocaine has stimulatory effect on TNF-α production by lymphocytes but inhibitory action on TNF-α production by monocyte/macrophages. In vitro cocaine treatment of monocyte-enriched preparations of mononuclear cells from normal donors resulted in suppression of cytokine production. A blood-brain barrier model was constructed using human brain microvascular endothelial cells. In this model mononuclear cell transmigration was correctly regulated by Th1 and Th2 cytokines and preferential migration of 'memory' T cells was inhibited by cocaethylene. TNF-α and cocaethylene increased HIV-1 titers in the brain- side of the model.

Original languageEnglish (US)
Pages (from-to)145-156
Number of pages12
JournalAdvances in Experimental Medicine and Biology
Volume402
StatePublished - 1996

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Lymphocytes
Macrophages
Blood-Brain Barrier
Cocaine
HIV-1
Monocytes
Tumor Necrosis Factor-alpha
Cytokines
Interleukin-10
Brain
Drug Evaluation
T-cells
Endothelial cells
Interleukin-12
Intravenous Administration
Immunosuppression
Leukocytes
Blood
Endothelial Cells
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Divergent effects of cocaine on cytokine production by lymphocytes and monocyte/macrophages : HIV-1 enhancement by cocaine within the blood-brain barrier. / Fiala, A. M.; Gan, X. H.; Newton, T.; Chiappelli, F.; Shapshak, P.; Kermani, V.; Kung, M. A.; Diagne, A.; Martinez, O.; Way, D.; Weinand, Martin E; Witte, Marlys H; Graves, M.

In: Advances in Experimental Medicine and Biology, Vol. 402, 1996, p. 145-156.

Research output: Contribution to journalArticle

Fiala, AM, Gan, XH, Newton, T, Chiappelli, F, Shapshak, P, Kermani, V, Kung, MA, Diagne, A, Martinez, O, Way, D, Weinand, ME, Witte, MH & Graves, M 1996, 'Divergent effects of cocaine on cytokine production by lymphocytes and monocyte/macrophages: HIV-1 enhancement by cocaine within the blood-brain barrier', Advances in Experimental Medicine and Biology, vol. 402, pp. 145-156.
Fiala, A. M. ; Gan, X. H. ; Newton, T. ; Chiappelli, F. ; Shapshak, P. ; Kermani, V. ; Kung, M. A. ; Diagne, A. ; Martinez, O. ; Way, D. ; Weinand, Martin E ; Witte, Marlys H ; Graves, M. / Divergent effects of cocaine on cytokine production by lymphocytes and monocyte/macrophages : HIV-1 enhancement by cocaine within the blood-brain barrier. In: Advances in Experimental Medicine and Biology. 1996 ; Vol. 402. pp. 145-156.
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AU - Graves, M.

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