Diversity and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in South Africa

Justin Hatting, S Patricia Stock, Selçuk Hazir

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A total of 1506 soil samples from different habitats in seven geographic regions of South Africa were evaluated for the presence of entomopathogenic nematodes (EPN). Nematodes were isolated from 5% of the samples. Among the steinernematids, four Steinernema sp. were recovered including Steinernema khoisanae and three new undescribed species. Although steinernematids were recovered from both humid subtropical and semiarid regions, this family accounted for 80% of EPN recovered from the semiarid climate zones characterised by sandy, acidic soils. Eight isolates of S. khoisanae were recovered from the Western Cape province. One of the new undescribed steinernematids (Steinernema sp. 1) was recovered only from the Free State and KwaZulu-Natal provinces where humid subtropical conditions prevail and soils are generally less acidic with higher clay content. A high level of adaptation, however, was noted with Steinernema sp. 2, which was recovered from a wide range of soil conditions and habitats ranging from semiarid (Western Cape province) to humid subtropical (KwaZulu-Natal province). A third undescribed steinernematid, Steinernema sp. 3, seemed better adapted to heavier soils with more than 80% of isolates recovered from fruit orchards in the Free State province. Heterorhabditis bacteriophora was the only heterorhabditid recovered during this survey. This species was particularly prevalent in four provinces ranging from humid subtropical to semiarid regions. Isolation of EPN directly from insect cadavers included Steinernema sp. 2 and one H. bacteriophora from an unidentified white grub (Scarabaeidae) cadaver (i.e., dual infection) and H. bacteriophora from the black vine weevil, Otiorhynchus sulcatus.

Original languageEnglish (US)
Pages (from-to)120-128
Number of pages9
JournalJournal of Invertebrate Pathology
Volume102
Issue number2
DOIs
StatePublished - Oct 2009

Fingerprint

Heterorhabditidae
Steinernematidae
Steinernema
entomopathogenic nematodes
nematode
South Africa
Heterorhabditis bacteriophora
Otiorhynchus sulcatus
semiarid region
soil
subtropical region
vine
Scarabaeidae
semiarid zones
habitat
orchard
acid soils
sandy soil
Africa
distribution

Keywords

  • Crops
  • Geographic regions
  • Insect-pathogenic nematodes
  • Natural habitats
  • South Africa
  • Survey

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Diversity and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in South Africa. / Hatting, Justin; Stock, S Patricia; Hazir, Selçuk.

In: Journal of Invertebrate Pathology, Vol. 102, No. 2, 10.2009, p. 120-128.

Research output: Contribution to journalArticle

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