Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses

Rob W. Briddon, Simon E. Bull, Imran Amin, Ali M. Idris, Shahid Mansoor, Ian D. Bedford, Poonam Dhawan, Narayan Rishi, Surender S. Siwatch, Aly M. Abdel-Salam, Judith K Brown, Yusuf Zafar, Peter G. Markham

Research output: Contribution to journalArticle

285 Citations (Scopus)

Abstract

DNA β molecules are symptom-modulating, single-stranded DNA satellites associated with monopartite begomoviruses (family Geminiviridae). Such molecules have thus far been shown to be associated with Ageratum yellow vein virus from Singapore and Cotton leaf curl Multan virus from Pakistan. Here, 26 additional DNA β molecules, associated with diverse plant species obtained from different geographical locations, were cloned and sequenced. These molecules were shown to be widespread in the Old World, where monopartite begomoviruses are known to occur. Analysis of the sequences revealed a highly conserved organization for DNA β molecules consisting of a single conserved open reading frame, an adenine-rich region, and a region of high sequence conservation [the satellite conserved region (SCR)]. The SCR contains a potential hairpin structure with the loop sequence TAA/GTATTAC; similar to the origins of replication of geminiviruses and nanoviruses. Two major groups of DNA β satellites were resolved by phylogenetic analyses. One group originated from hosts within the Malvaceae and the second from a more diverse group of plants within the Solanaceae and Compositae. Within the two clusters, DNA β molecules showed relatedness based both on host and geographic origin. These findings strongly support coadaptation of DNA β molecules with their respective helper begomoviruses.

Original languageEnglish (US)
Pages (from-to)106-121
Number of pages16
JournalVirology
Volume312
Issue number1
DOIs
StatePublished - Jul 20 2003

Fingerprint

Begomovirus
Satellite DNA
Geminiviridae
DNA
Nanovirus
Ageratum
Malvaceae
Solanaceae
Viruses
Asteraceae
Replication Origin
Single-Stranded DNA
Pakistan
Singapore
Adenine
Open Reading Frames
Sequence Analysis
Veins

Keywords

  • Begomovirus
  • Diversity
  • DNA β
  • Evolution
  • Geminivirus
  • Satellite

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Briddon, R. W., Bull, S. E., Amin, I., Idris, A. M., Mansoor, S., Bedford, I. D., ... Markham, P. G. (2003). Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. Virology, 312(1), 106-121. https://doi.org/10.1016/S0042-6822(03)00200-9

Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. / Briddon, Rob W.; Bull, Simon E.; Amin, Imran; Idris, Ali M.; Mansoor, Shahid; Bedford, Ian D.; Dhawan, Poonam; Rishi, Narayan; Siwatch, Surender S.; Abdel-Salam, Aly M.; Brown, Judith K; Zafar, Yusuf; Markham, Peter G.

In: Virology, Vol. 312, No. 1, 20.07.2003, p. 106-121.

Research output: Contribution to journalArticle

Briddon, RW, Bull, SE, Amin, I, Idris, AM, Mansoor, S, Bedford, ID, Dhawan, P, Rishi, N, Siwatch, SS, Abdel-Salam, AM, Brown, JK, Zafar, Y & Markham, PG 2003, 'Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses', Virology, vol. 312, no. 1, pp. 106-121. https://doi.org/10.1016/S0042-6822(03)00200-9
Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID et al. Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. Virology. 2003 Jul 20;312(1):106-121. https://doi.org/10.1016/S0042-6822(03)00200-9
Briddon, Rob W. ; Bull, Simon E. ; Amin, Imran ; Idris, Ali M. ; Mansoor, Shahid ; Bedford, Ian D. ; Dhawan, Poonam ; Rishi, Narayan ; Siwatch, Surender S. ; Abdel-Salam, Aly M. ; Brown, Judith K ; Zafar, Yusuf ; Markham, Peter G. / Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. In: Virology. 2003 ; Vol. 312, No. 1. pp. 106-121.
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