Diversity of voltage-dependent K+ channels in human pulmonary artery smooth muscle cell

Oleksandr Platoshyn, Carmelle V. Remillard, Ivana Fantozzi, Mehran Mandegar, Tiffany T. Sison, Shen Zhang, Elyssa Burg, Jason Yuan

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Electrical excitability, which plays an important role in excitation-contraction coupling in the pulmonary vasculature, is regulated by transmembrane ion flux in pulmonary artery smooth muscle cells (PASMC). This study examined the heterogeneous nature of native voltage-dependent K + channels in human PASMC. Both voltage-gated K+ (K v) currents and Ca2+-activated K+ (K ca) currents were observed and characterized. In cell-attached patches of PASMC bathed in Ca2+-containing solutions, depolarization elicited a wide range of K+ unitary conductances (6-290 pS). When cells were dialyzed with Ca2+-free and K+-containing solutions, depolarization elicited four components of Kv currents in PASMC based on the kinetics of current activation and inactivation. Using RT-PCR, we detected transcripts of 1) 22 Kv channel α-subunits (Kv1.1-1.7, Kv1.10, Kv2.1, Kv3.1, Kv3.3-3.4, Kv4.1-4.2, Kv5.1, Kv 6.1-6.3, Kv9.1, Kv9.3, Kv10.1, and K v11.1), 2) three Kv channel β-subunits (K vβ1-3), 3) four Kca channel α-subunits (Slo-α1 and SK2-SK4), and 4) four Kca channel β-subunits (Kcaβ1-4). Our results show that human PASMC exhibit a variety of voltage-dependent K+ currents with variable kinetics and conductances, which may result from various unique combinations of α- and β-subunits forming the native channels. Functional expression of these channels plays a critical role in the regulation of membrane potential, cytoplasmic Ca2+, and pulmonary vasomotor tone.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume287
Issue number1 31-1
DOIs
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Pulmonary Artery
Smooth Muscle Myocytes
Excitation Contraction Coupling
Lung
Membrane Potentials
Ions
Polymerase Chain Reaction

Keywords

  • Calcium
  • Calcium-activated potassium channel
  • Heterogeneity
  • Membrane potential
  • Proliferation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Diversity of voltage-dependent K+ channels in human pulmonary artery smooth muscle cell. / Platoshyn, Oleksandr; Remillard, Carmelle V.; Fantozzi, Ivana; Mandegar, Mehran; Sison, Tiffany T.; Zhang, Shen; Burg, Elyssa; Yuan, Jason.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 287, No. 1 31-1, 07.2004.

Research output: Contribution to journalArticle

Platoshyn, Oleksandr ; Remillard, Carmelle V. ; Fantozzi, Ivana ; Mandegar, Mehran ; Sison, Tiffany T. ; Zhang, Shen ; Burg, Elyssa ; Yuan, Jason. / Diversity of voltage-dependent K+ channels in human pulmonary artery smooth muscle cell. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2004 ; Vol. 287, No. 1 31-1.
@article{96c4e0b2187d4dc9bdf9cf06c66d7e5d,
title = "Diversity of voltage-dependent K+ channels in human pulmonary artery smooth muscle cell",
abstract = "Electrical excitability, which plays an important role in excitation-contraction coupling in the pulmonary vasculature, is regulated by transmembrane ion flux in pulmonary artery smooth muscle cells (PASMC). This study examined the heterogeneous nature of native voltage-dependent K + channels in human PASMC. Both voltage-gated K+ (K v) currents and Ca2+-activated K+ (K ca) currents were observed and characterized. In cell-attached patches of PASMC bathed in Ca2+-containing solutions, depolarization elicited a wide range of K+ unitary conductances (6-290 pS). When cells were dialyzed with Ca2+-free and K+-containing solutions, depolarization elicited four components of Kv currents in PASMC based on the kinetics of current activation and inactivation. Using RT-PCR, we detected transcripts of 1) 22 Kv channel α-subunits (Kv1.1-1.7, Kv1.10, Kv2.1, Kv3.1, Kv3.3-3.4, Kv4.1-4.2, Kv5.1, Kv 6.1-6.3, Kv9.1, Kv9.3, Kv10.1, and K v11.1), 2) three Kv channel β-subunits (K vβ1-3), 3) four Kca channel α-subunits (Slo-α1 and SK2-SK4), and 4) four Kca channel β-subunits (Kcaβ1-4). Our results show that human PASMC exhibit a variety of voltage-dependent K+ currents with variable kinetics and conductances, which may result from various unique combinations of α- and β-subunits forming the native channels. Functional expression of these channels plays a critical role in the regulation of membrane potential, cytoplasmic Ca2+, and pulmonary vasomotor tone.",
keywords = "Calcium, Calcium-activated potassium channel, Heterogeneity, Membrane potential, Proliferation",
author = "Oleksandr Platoshyn and Remillard, {Carmelle V.} and Ivana Fantozzi and Mehran Mandegar and Sison, {Tiffany T.} and Shen Zhang and Elyssa Burg and Jason Yuan",
year = "2004",
month = "7",
doi = "10.1152/ajplung.00438.2003",
language = "English (US)",
volume = "287",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "1 31-1",

}

TY - JOUR

T1 - Diversity of voltage-dependent K+ channels in human pulmonary artery smooth muscle cell

AU - Platoshyn, Oleksandr

AU - Remillard, Carmelle V.

AU - Fantozzi, Ivana

AU - Mandegar, Mehran

AU - Sison, Tiffany T.

AU - Zhang, Shen

AU - Burg, Elyssa

AU - Yuan, Jason

PY - 2004/7

Y1 - 2004/7

N2 - Electrical excitability, which plays an important role in excitation-contraction coupling in the pulmonary vasculature, is regulated by transmembrane ion flux in pulmonary artery smooth muscle cells (PASMC). This study examined the heterogeneous nature of native voltage-dependent K + channels in human PASMC. Both voltage-gated K+ (K v) currents and Ca2+-activated K+ (K ca) currents were observed and characterized. In cell-attached patches of PASMC bathed in Ca2+-containing solutions, depolarization elicited a wide range of K+ unitary conductances (6-290 pS). When cells were dialyzed with Ca2+-free and K+-containing solutions, depolarization elicited four components of Kv currents in PASMC based on the kinetics of current activation and inactivation. Using RT-PCR, we detected transcripts of 1) 22 Kv channel α-subunits (Kv1.1-1.7, Kv1.10, Kv2.1, Kv3.1, Kv3.3-3.4, Kv4.1-4.2, Kv5.1, Kv 6.1-6.3, Kv9.1, Kv9.3, Kv10.1, and K v11.1), 2) three Kv channel β-subunits (K vβ1-3), 3) four Kca channel α-subunits (Slo-α1 and SK2-SK4), and 4) four Kca channel β-subunits (Kcaβ1-4). Our results show that human PASMC exhibit a variety of voltage-dependent K+ currents with variable kinetics and conductances, which may result from various unique combinations of α- and β-subunits forming the native channels. Functional expression of these channels plays a critical role in the regulation of membrane potential, cytoplasmic Ca2+, and pulmonary vasomotor tone.

AB - Electrical excitability, which plays an important role in excitation-contraction coupling in the pulmonary vasculature, is regulated by transmembrane ion flux in pulmonary artery smooth muscle cells (PASMC). This study examined the heterogeneous nature of native voltage-dependent K + channels in human PASMC. Both voltage-gated K+ (K v) currents and Ca2+-activated K+ (K ca) currents were observed and characterized. In cell-attached patches of PASMC bathed in Ca2+-containing solutions, depolarization elicited a wide range of K+ unitary conductances (6-290 pS). When cells were dialyzed with Ca2+-free and K+-containing solutions, depolarization elicited four components of Kv currents in PASMC based on the kinetics of current activation and inactivation. Using RT-PCR, we detected transcripts of 1) 22 Kv channel α-subunits (Kv1.1-1.7, Kv1.10, Kv2.1, Kv3.1, Kv3.3-3.4, Kv4.1-4.2, Kv5.1, Kv 6.1-6.3, Kv9.1, Kv9.3, Kv10.1, and K v11.1), 2) three Kv channel β-subunits (K vβ1-3), 3) four Kca channel α-subunits (Slo-α1 and SK2-SK4), and 4) four Kca channel β-subunits (Kcaβ1-4). Our results show that human PASMC exhibit a variety of voltage-dependent K+ currents with variable kinetics and conductances, which may result from various unique combinations of α- and β-subunits forming the native channels. Functional expression of these channels plays a critical role in the regulation of membrane potential, cytoplasmic Ca2+, and pulmonary vasomotor tone.

KW - Calcium

KW - Calcium-activated potassium channel

KW - Heterogeneity

KW - Membrane potential

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=2942726402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942726402&partnerID=8YFLogxK

U2 - 10.1152/ajplung.00438.2003

DO - 10.1152/ajplung.00438.2003

M3 - Article

C2 - 15047570

AN - SCOPUS:2942726402

VL - 287

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 1 31-1

ER -