Diversity-Oriented Combinatorial Biosynthesis of Hybrid Polyketide Scaffolds from Azaphilone and Benzenediol Lactone Biosynthons

Jing Bai, Yuanyuan Lu, Ya Ming Xu, Wei Zhang, Ming Chen, Min Lin, Leslie Gunatilaka, Yuquan Xu, Istvan Molnar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Two disparate polyketide families, the benzenediol lactones and the azaphilones, are produced by fungi using iterative polyketide synthase (iPKS) enzymes consisting of collaborating partner subunits. Exploitation of this common biosynthetic logic using iPKS subunit shuffling allowed the diversity-oriented combinatorial biosynthesis of unprecedented polyketide scaffolds new to nature, bearing structural motifs from both of these orthogonal natural product families. Starter unit acyltransferase domain replacements proved necessary but not sufficient to guarantee communication between iPKS subunits.

Original languageEnglish (US)
Pages (from-to)1262-1265
Number of pages4
JournalOrganic Letters
Volume18
Issue number6
DOIs
StatePublished - Mar 18 2016

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starters
Polyketide Synthases
Polyketides
biosynthesis
fungi
Biosynthesis
Lactones
exploitation
Scaffolds
logic
enzymes
communication
Bearings (structural)
products
Acyltransferases
Starters
Biological Products
Fungi
Communication
Enzymes

ASJC Scopus subject areas

  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Biochemistry

Cite this

Diversity-Oriented Combinatorial Biosynthesis of Hybrid Polyketide Scaffolds from Azaphilone and Benzenediol Lactone Biosynthons. / Bai, Jing; Lu, Yuanyuan; Xu, Ya Ming; Zhang, Wei; Chen, Ming; Lin, Min; Gunatilaka, Leslie; Xu, Yuquan; Molnar, Istvan.

In: Organic Letters, Vol. 18, No. 6, 18.03.2016, p. 1262-1265.

Research output: Contribution to journalArticle

Bai, Jing ; Lu, Yuanyuan ; Xu, Ya Ming ; Zhang, Wei ; Chen, Ming ; Lin, Min ; Gunatilaka, Leslie ; Xu, Yuquan ; Molnar, Istvan. / Diversity-Oriented Combinatorial Biosynthesis of Hybrid Polyketide Scaffolds from Azaphilone and Benzenediol Lactone Biosynthons. In: Organic Letters. 2016 ; Vol. 18, No. 6. pp. 1262-1265.
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