DNA and RNA polymerase/DNA complex imaged by scanning force microscopy

Influence of molecular-scale friction

Frederic Zenhausern, M. Adrian, B. T. Heggeler-Bordier, L. M. Eng, P. Descouts

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Reproducible images of DNA and the interaction of RNA polymerase with supercoiled DNA on mica have been obtained by scanning force microscopy (SFM) in air. This technique allowed visualization of the assembly of distinct nucleoprotein complexes at specific sites which initiate DNA replication. Transmission electron microscopic controls were performed on the carbon- coated replica of the same specimens previously scanned by scanning force microscope. Image contrast formation has been investigated and lateral friction forces have been qualitatively characterized with SFM on DNA-covered samples.

Original languageEnglish (US)
Pages (from-to)212-217
Number of pages6
JournalScanning
Volume14
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

RNA
Atomic force microscopy
DNA
friction
deoxyribonucleic acid
Friction
microscopy
scanning
Mica
image contrast
mica
replicas
Microscopes
Visualization
assembly
microscopes
Scanning
Carbon
Electrons
carbon

ASJC Scopus subject areas

  • Instrumentation

Cite this

Zenhausern, F., Adrian, M., Heggeler-Bordier, B. T., Eng, L. M., & Descouts, P. (1992). DNA and RNA polymerase/DNA complex imaged by scanning force microscopy: Influence of molecular-scale friction. Scanning, 14(4), 212-217.

DNA and RNA polymerase/DNA complex imaged by scanning force microscopy : Influence of molecular-scale friction. / Zenhausern, Frederic; Adrian, M.; Heggeler-Bordier, B. T.; Eng, L. M.; Descouts, P.

In: Scanning, Vol. 14, No. 4, 1992, p. 212-217.

Research output: Contribution to journalArticle

Zenhausern, F, Adrian, M, Heggeler-Bordier, BT, Eng, LM & Descouts, P 1992, 'DNA and RNA polymerase/DNA complex imaged by scanning force microscopy: Influence of molecular-scale friction', Scanning, vol. 14, no. 4, pp. 212-217.
Zenhausern, Frederic ; Adrian, M. ; Heggeler-Bordier, B. T. ; Eng, L. M. ; Descouts, P. / DNA and RNA polymerase/DNA complex imaged by scanning force microscopy : Influence of molecular-scale friction. In: Scanning. 1992 ; Vol. 14, No. 4. pp. 212-217.
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