DNA and RNA polymerase/DNA complex imaged by scanning force microscopy: Influence of molecular‐scale friction

F. Zenhausern, M. Adrian, B. Ten Heggeler‐Bordier, L. M. Eng, P. Descouts

Research output: Contribution to journalArticle

72 Scopus citations


Reproducible images of DNA and the interaction of RNA polymerase with supercoiled DNA on mica have been obtained by scanning force microscopy (SFM) in air. This technique allowed visualization of the assembly of distinct nucleoprotein complexes at specific sites which initiate DNA replication. Transmission electron microscopic controls were performed on the carbon‐coated replica of the same specimens previously scanned by scanning force microscope. Image contrast formation has been investigated and lateral friction forces have been qualitatively characterized with SFM on DNA‐covered samples.

Original languageEnglish (US)
Pages (from-to)212-217
Number of pages6
Issue number4
StatePublished - 1992
Externally publishedYes


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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