DNA damage and mismatch repair pathway in lung ischemia and reperfusion injury

Pramod Bonde, Daqing Gao, Lei Chen, Liliana Moreno-Vinasco, Jeff Jacobson, Joe GN Garcia, Chiming Wei

Research output: Contribution to journalArticle

Abstract

Background: Oxidative damage induced by reperfusion is responsible for increased morbidity and mortality following lung transplantation. A stable and deleterious DNA adduct, 8-oxogaunine (8-oxoG) results due to oxidative DNA damage. Mut-Y homologue (MYH) is a DNA repair enzyme promoting DNA reconstruction through the mismatch repair pathway to repair 8-oxoG lesion. We investigated the role of DNA mismatch repair pathway mediated by MYH in the setting of lung ischemia and reperfusion. Methods: Left lungs of the adult Sprague Dawley rats were subjected to 1 h ischemia and 2 and 4 h reperfusion. Un-operated animals served as controls. Quantification of 8-oxoG was performed using immunohistochemistry (IHC) and MYH was analyzed by Western blot. Apoptosis was assessed by caspase-3 levels. Results: Indices of inflammation and permeability were raised in both reperfusion groups. There was significant increase in DNA damage as reflected by positive 8-oxoG staining in 2 h (22% increase) and 4 h reperfusion (31% increase) compared to control (p < 0.01). MYH staining by IHC was significantly reduced in 2 and 4 h reperfusion compared to controls (p < 0.05). Down regulation of DNA repair enzyme (MYH) was mirrored functionally by decreased protein levels in lung tissues subjected to reperfusion compared to controls. Increasing apoptosis was detected in the reperfusion groups as reflected by caspase-3 IHC and protein estimation by Western blot. Conclusion: Reperfusion leads to increased DNA damage and down regulation of DNA mismatch repair pathway in a model of ischemia and reperfusion in lungs. Gene therapy targeted at this pathway may prove an attractive therapeutic intervention to reduce reperfusion injury in lung transplantation.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalJournal of Cardiothoracic-Renal Research
Volume1
Issue number2
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

DNA Mismatch Repair
Lung Injury
Reperfusion Injury
DNA Damage
Reperfusion
DNA Repair Enzymes
Lung
Ischemia
Lung Transplantation
Immunohistochemistry
Caspase 3
Down-Regulation
Western Blotting
Apoptosis
Staining and Labeling
DNA Adducts
Genetic Therapy
Sprague Dawley Rats
Permeability
Proteins

Keywords

  • 8-oxoG
  • Apoptosis
  • DNA damage
  • DNA repair
  • Ischemia-reperfusion injury
  • Lung transplantation
  • MYH
  • Oxidative stress

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Nephrology
  • Pulmonary and Respiratory Medicine

Cite this

DNA damage and mismatch repair pathway in lung ischemia and reperfusion injury. / Bonde, Pramod; Gao, Daqing; Chen, Lei; Moreno-Vinasco, Liliana; Jacobson, Jeff; Garcia, Joe GN; Wei, Chiming.

In: Journal of Cardiothoracic-Renal Research, Vol. 1, No. 2, 09.2006, p. 147-152.

Research output: Contribution to journalArticle

Bonde, Pramod ; Gao, Daqing ; Chen, Lei ; Moreno-Vinasco, Liliana ; Jacobson, Jeff ; Garcia, Joe GN ; Wei, Chiming. / DNA damage and mismatch repair pathway in lung ischemia and reperfusion injury. In: Journal of Cardiothoracic-Renal Research. 2006 ; Vol. 1, No. 2. pp. 147-152.
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abstract = "Background: Oxidative damage induced by reperfusion is responsible for increased morbidity and mortality following lung transplantation. A stable and deleterious DNA adduct, 8-oxogaunine (8-oxoG) results due to oxidative DNA damage. Mut-Y homologue (MYH) is a DNA repair enzyme promoting DNA reconstruction through the mismatch repair pathway to repair 8-oxoG lesion. We investigated the role of DNA mismatch repair pathway mediated by MYH in the setting of lung ischemia and reperfusion. Methods: Left lungs of the adult Sprague Dawley rats were subjected to 1 h ischemia and 2 and 4 h reperfusion. Un-operated animals served as controls. Quantification of 8-oxoG was performed using immunohistochemistry (IHC) and MYH was analyzed by Western blot. Apoptosis was assessed by caspase-3 levels. Results: Indices of inflammation and permeability were raised in both reperfusion groups. There was significant increase in DNA damage as reflected by positive 8-oxoG staining in 2 h (22{\%} increase) and 4 h reperfusion (31{\%} increase) compared to control (p < 0.01). MYH staining by IHC was significantly reduced in 2 and 4 h reperfusion compared to controls (p < 0.05). Down regulation of DNA repair enzyme (MYH) was mirrored functionally by decreased protein levels in lung tissues subjected to reperfusion compared to controls. Increasing apoptosis was detected in the reperfusion groups as reflected by caspase-3 IHC and protein estimation by Western blot. Conclusion: Reperfusion leads to increased DNA damage and down regulation of DNA mismatch repair pathway in a model of ischemia and reperfusion in lungs. Gene therapy targeted at this pathway may prove an attractive therapeutic intervention to reduce reperfusion injury in lung transplantation.",
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AU - Bonde, Pramod

AU - Gao, Daqing

AU - Chen, Lei

AU - Moreno-Vinasco, Liliana

AU - Jacobson, Jeff

AU - Garcia, Joe GN

AU - Wei, Chiming

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N2 - Background: Oxidative damage induced by reperfusion is responsible for increased morbidity and mortality following lung transplantation. A stable and deleterious DNA adduct, 8-oxogaunine (8-oxoG) results due to oxidative DNA damage. Mut-Y homologue (MYH) is a DNA repair enzyme promoting DNA reconstruction through the mismatch repair pathway to repair 8-oxoG lesion. We investigated the role of DNA mismatch repair pathway mediated by MYH in the setting of lung ischemia and reperfusion. Methods: Left lungs of the adult Sprague Dawley rats were subjected to 1 h ischemia and 2 and 4 h reperfusion. Un-operated animals served as controls. Quantification of 8-oxoG was performed using immunohistochemistry (IHC) and MYH was analyzed by Western blot. Apoptosis was assessed by caspase-3 levels. Results: Indices of inflammation and permeability were raised in both reperfusion groups. There was significant increase in DNA damage as reflected by positive 8-oxoG staining in 2 h (22% increase) and 4 h reperfusion (31% increase) compared to control (p < 0.01). MYH staining by IHC was significantly reduced in 2 and 4 h reperfusion compared to controls (p < 0.05). Down regulation of DNA repair enzyme (MYH) was mirrored functionally by decreased protein levels in lung tissues subjected to reperfusion compared to controls. Increasing apoptosis was detected in the reperfusion groups as reflected by caspase-3 IHC and protein estimation by Western blot. Conclusion: Reperfusion leads to increased DNA damage and down regulation of DNA mismatch repair pathway in a model of ischemia and reperfusion in lungs. Gene therapy targeted at this pathway may prove an attractive therapeutic intervention to reduce reperfusion injury in lung transplantation.

AB - Background: Oxidative damage induced by reperfusion is responsible for increased morbidity and mortality following lung transplantation. A stable and deleterious DNA adduct, 8-oxogaunine (8-oxoG) results due to oxidative DNA damage. Mut-Y homologue (MYH) is a DNA repair enzyme promoting DNA reconstruction through the mismatch repair pathway to repair 8-oxoG lesion. We investigated the role of DNA mismatch repair pathway mediated by MYH in the setting of lung ischemia and reperfusion. Methods: Left lungs of the adult Sprague Dawley rats were subjected to 1 h ischemia and 2 and 4 h reperfusion. Un-operated animals served as controls. Quantification of 8-oxoG was performed using immunohistochemistry (IHC) and MYH was analyzed by Western blot. Apoptosis was assessed by caspase-3 levels. Results: Indices of inflammation and permeability were raised in both reperfusion groups. There was significant increase in DNA damage as reflected by positive 8-oxoG staining in 2 h (22% increase) and 4 h reperfusion (31% increase) compared to control (p < 0.01). MYH staining by IHC was significantly reduced in 2 and 4 h reperfusion compared to controls (p < 0.05). Down regulation of DNA repair enzyme (MYH) was mirrored functionally by decreased protein levels in lung tissues subjected to reperfusion compared to controls. Increasing apoptosis was detected in the reperfusion groups as reflected by caspase-3 IHC and protein estimation by Western blot. Conclusion: Reperfusion leads to increased DNA damage and down regulation of DNA mismatch repair pathway in a model of ischemia and reperfusion in lungs. Gene therapy targeted at this pathway may prove an attractive therapeutic intervention to reduce reperfusion injury in lung transplantation.

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