DNA methylation among firefighters

Jin Zhou, Timothy G. Jenkins, Alesia M. Jung, Kyoung Sook Jeong, Jing Zhai, Elizabeth T Jacobs, Stephanie Griffin, Devi Dearmon-Moore, Sally R. Littau, Wayne F. Peate, Nathan Ellis, Michael P Lance, Yin Chen, Jefferey L Burgess

Research output: Contribution to journalArticle

Abstract

Firefighters are exposed to carcinogens and have elevated cancer rates. We hypothesized that occupational exposures in firefighters would lead to DNA methylation changes associated with activation of cancer pathways and increased cancer risk. To address this hypothesis, we collected peripheral blood samples from 45 incumbent and 41 new recruit nonsmoking male firefighters and analyzed the samples for DNA methylation using an Illumina Methylation EPIC 850k chip. Adjusting for age and ethnicity, we performed: 1) genome-wide differential methylation analysis; 2) genome-wide prediction for firefighter status (incumbent or new recruit) and years of service; and 3) Ingenuity Pathway Analysis (IPA). Four CpGs, including three in the YIPF6, MPST, and PCED1B genes, demonstrated above 1.5-fold statistically significant differential methylation after Bonferroni correction. Genome-wide methylation predicted with high accuracy incumbent and new recruit status as well as years of service among incumbent firefighters. Using IPA, the top pathways with more than 5 gene members annotated from differentially methylated probes included Sirtuin signaling pathway, p53 signaling, and 5’ AMP-activated protein kinase (AMPK) signaling. These DNA methylation findings suggest potential cellular mechanisms associated with increased cancer risk in firefighters.

Original languageEnglish (US)
Article numbere0214282
JournalPloS one
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2019

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fire fighters
Firefighters
DNA methylation
DNA Methylation
Methylation
Genes
methylation
neoplasms
Genome
genome
Neoplasms
AMP-Activated Protein Kinases
occupational exposure
new status
AMP-activated protein kinase
Carcinogens
carcinogens
Occupational Exposure
nationalities and ethnic groups
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

DNA methylation among firefighters. / Zhou, Jin; Jenkins, Timothy G.; Jung, Alesia M.; Jeong, Kyoung Sook; Zhai, Jing; Jacobs, Elizabeth T; Griffin, Stephanie; Dearmon-Moore, Devi; Littau, Sally R.; Peate, Wayne F.; Ellis, Nathan; Lance, Michael P; Chen, Yin; Burgess, Jefferey L.

In: PloS one, Vol. 14, No. 3, e0214282, 01.03.2019.

Research output: Contribution to journalArticle

Zhou, J, Jenkins, TG, Jung, AM, Jeong, KS, Zhai, J, Jacobs, ET, Griffin, S, Dearmon-Moore, D, Littau, SR, Peate, WF, Ellis, N, Lance, MP, Chen, Y & Burgess, JL 2019, 'DNA methylation among firefighters' PloS one, vol. 14, no. 3, e0214282. https://doi.org/10.1371/journal.pone.0214282
Zhou J, Jenkins TG, Jung AM, Jeong KS, Zhai J, Jacobs ET et al. DNA methylation among firefighters. PloS one. 2019 Mar 1;14(3). e0214282. https://doi.org/10.1371/journal.pone.0214282
Zhou, Jin ; Jenkins, Timothy G. ; Jung, Alesia M. ; Jeong, Kyoung Sook ; Zhai, Jing ; Jacobs, Elizabeth T ; Griffin, Stephanie ; Dearmon-Moore, Devi ; Littau, Sally R. ; Peate, Wayne F. ; Ellis, Nathan ; Lance, Michael P ; Chen, Yin ; Burgess, Jefferey L. / DNA methylation among firefighters. In: PloS one. 2019 ; Vol. 14, No. 3.
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