DNA vaccination of mice to immunize against Coccidioides immitis

E. M. Siegel, Lisa Shubitz, C. R. Zimmermann, K. I. Orsborn, D. Pappagianis, Charles R Sterling, John N Galgiani

Research output: Contribution to journalArticle

Abstract

Life-long immunity follows most coccidioidal infections and vaccination with killed spherule vaccine protects mice from later infection. Indirect evidence implicates a proline-rich antigen (PRA) as a stimulus of cellular immunity. To study this directly, a 597 bp PCR amplimer encoding PRA was inserted into plasmid vectors VR1012 and VR1020 (Vical, San Diego CA). DNA sequencing verified orientation and reading frame. Female Swiss-Webster mice, 6-8 weeks old, received 100 μg i.m. of plasmid DNA. ELISA with recombinant PRA was used to detect a humoral response. After a single vaccination with either vector, PRA-specific IgG was detected by 2 wks (mean±SEM OD of 0.347±0.038 versus 0.128±0.004 for vector control mice, serum diluted 1:320, p<0.004). Antibodies were elevated for over 2 months. Vaccination repeated at 4 wks boosted IgG levels in 6 of 7 mice studied. In a preliminary study of intranasal C. immitis infection, 8 surviving mice had higher preinfection IgG levels in serum diluted 1:640 (0.939 ± 0.162) as compared to 4 mice who died before day 21 (0.494 ± 0.167). DNA vaccination will be a useful tool for studies of coccidioidal immunity.

Original languageEnglish (US)
Pages (from-to)357
Number of pages1
JournalClinical Infectious Diseases
Volume25
Issue number2
StatePublished - 1997
Externally publishedYes

Fingerprint

Coccidioides
Vaccination
Proline
DNA
Antigens
Immunoglobulin G
Immunity
Plasmids
Coccidioidomycosis
Reading Frames
Inactivated Vaccines
Infection
Serum
DNA Sequence Analysis
Cellular Immunity
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

DNA vaccination of mice to immunize against Coccidioides immitis. / Siegel, E. M.; Shubitz, Lisa; Zimmermann, C. R.; Orsborn, K. I.; Pappagianis, D.; Sterling, Charles R; Galgiani, John N.

In: Clinical Infectious Diseases, Vol. 25, No. 2, 1997, p. 357.

Research output: Contribution to journalArticle

Siegel, EM, Shubitz, L, Zimmermann, CR, Orsborn, KI, Pappagianis, D, Sterling, CR & Galgiani, JN 1997, 'DNA vaccination of mice to immunize against Coccidioides immitis', Clinical Infectious Diseases, vol. 25, no. 2, pp. 357.
Siegel EM, Shubitz L, Zimmermann CR, Orsborn KI, Pappagianis D, Sterling CR et al. DNA vaccination of mice to immunize against Coccidioides immitis. Clinical Infectious Diseases. 1997;25(2):357.
Siegel, E. M. ; Shubitz, Lisa ; Zimmermann, C. R. ; Orsborn, K. I. ; Pappagianis, D. ; Sterling, Charles R ; Galgiani, John N. / DNA vaccination of mice to immunize against Coccidioides immitis. In: Clinical Infectious Diseases. 1997 ; Vol. 25, No. 2. pp. 357.
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