Dodecafluoropentane emulsion decreases infarct volume in a rabbit ischemic stroke model

William C. Culp, Sean D. Woods, Robert D. Skinner, Aliza T. Brown, John D. Lowery, Jennifer L.H. Johnson, Evan C. Unger, Leah J. Hennings, Michael J. Borrelli, Paula K. Roberson

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Purpose: To assess the efficacy of dodecafluoropentane emulsion (DDFPe), a nanodroplet emulsion with significant oxygen transport potential, in decreasing infarct volume in an insoluble-emboli rabbit stroke model. Materials And Methods: New Zealand White rabbits (N = 64; weight, 5.1 ± 0.50 kg) underwent angiography and received embolic spheres in occluded internal carotid artery branches. Rabbits were randomly assigned to groups in 4-hour and 7-hour studies. Four-hour groups included control (n = 7, embolized without treatment) and DDFPe treatment 30 minutes before stroke (n = 7), at stroke onset (n = 8), and 30 minutes (n = 5), 1 hour (n = 7), 2 hours (n = 5), or 3 hours after stroke (n = 6). Seven-hour groups included control (n = 6) and DDFPe at 1 hour (n = 8) and 6 hours after stroke (n = 5). DDFPe dose was a 2% weight/volume intravenous injection (0.6 mL/kg) repeated every 90 minutes as time allowed. After euthanasia, infarct volume was determined by vital stains on brain sections. Results: At 4 hours, median infarct volume decreased for all DDFPe treatment times (pretreatment, 0.30% [P = .004]; onset, 0.20% [P = .004]; 30 min, 0.35% [P = .009]; 1 h, 0.30% [P = .01]; 2 h, 0.40% [P = .009]; and 3 h, 0.25% [P = .003]) compared with controls (3.20%). At 7 hours, median infarct volume decreased with treatment at 1 hour (0.25%; P = .007) but not at 6 hours (1.4%; P = .49) compared with controls (2.2%). Conclusions: Intravenous DDFPe in an animal model decreases infarct volumes and protects brain tissue from ischemia, justifying further investigation.

Original languageEnglish (US)
Pages (from-to)116-121
Number of pages6
JournalJournal of Vascular and Interventional Radiology
Volume23
Issue number1
DOIs
StatePublished - Jan 1 2012

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Dodecafluoropentane emulsion decreases infarct volume in a rabbit ischemic stroke model'. Together they form a unique fingerprint.

  • Cite this

    Culp, W. C., Woods, S. D., Skinner, R. D., Brown, A. T., Lowery, J. D., Johnson, J. L. H., Unger, E. C., Hennings, L. J., Borrelli, M. J., & Roberson, P. K. (2012). Dodecafluoropentane emulsion decreases infarct volume in a rabbit ischemic stroke model. Journal of Vascular and Interventional Radiology, 23(1), 116-121. https://doi.org/10.1016/j.jvir.2011.10.001