Domain swapping in allosteric modulation of DNA specificity

Chad K. Park, Hemant K. Joshi, Alka Agrawal, M. Imran Ghare, Elizabeth J. Little, Pete W. Dunten, Jurate Bitinaite, Nancy C Horton

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

SgrAI is a type IIF restriction endonuclease that cuts an unusually long recognition sequence and exhibits allosteric selfmodulation of cleavage activity and sequence specificity. Previous studies have shown that DNA bound dimers of SgrAI oligomerize into an activated form with higher DNA cleavage rates, although previously determined crystal structures of SgrAI bound to DNA show only the DNA bound dimer. A new crystal structure of the type II restriction endonuclease SgrAI bound to DNA and Ca2+ is now presented, which shows the close association of two DNA bound SgrAI dimers. This tetrameric form is unlike those of the homologous enzymes Cfr10I and NgoMIV and is formed by the swapping of the amino-terminal 24 amino acid residues. Two mutations predicted to destabilize the swapped form of SgrAI, P27W and P27G, have been made and shown to eliminate both the oligomerization of the DNA bound SgrAI dimers as well as the allosteric stimulation of DNA cleavage by SgrAI. A mechanism involving domain swapping is proposed to explain the unusual allosteric properties of SgrAI via association of the domain swapped tetramer of SgrAI bound to DNA into higher order oligomers.

Original languageEnglish (US)
Article numbere1000554
JournalPLoS Biology
Volume8
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

Modulation
DNA
Dimers
DNA Cleavage
restriction endonucleases
Type II Site Specific Deoxyribonucleases
crystal structure
Crystal structure
Association reactions
DNA Restriction Enzymes
Oligomerization
Oligomers
Amino Acids
Mutation
Enzymes
mutation
calcium
amino acids
enzymes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Park, C. K., Joshi, H. K., Agrawal, A., Ghare, M. I., Little, E. J., Dunten, P. W., ... Horton, N. C. (2010). Domain swapping in allosteric modulation of DNA specificity. PLoS Biology, 8(12), [e1000554]. https://doi.org/10.1371/journal.pbio.1000554

Domain swapping in allosteric modulation of DNA specificity. / Park, Chad K.; Joshi, Hemant K.; Agrawal, Alka; Ghare, M. Imran; Little, Elizabeth J.; Dunten, Pete W.; Bitinaite, Jurate; Horton, Nancy C.

In: PLoS Biology, Vol. 8, No. 12, e1000554, 12.2010.

Research output: Contribution to journalArticle

Park, CK, Joshi, HK, Agrawal, A, Ghare, MI, Little, EJ, Dunten, PW, Bitinaite, J & Horton, NC 2010, 'Domain swapping in allosteric modulation of DNA specificity', PLoS Biology, vol. 8, no. 12, e1000554. https://doi.org/10.1371/journal.pbio.1000554
Park CK, Joshi HK, Agrawal A, Ghare MI, Little EJ, Dunten PW et al. Domain swapping in allosteric modulation of DNA specificity. PLoS Biology. 2010 Dec;8(12). e1000554. https://doi.org/10.1371/journal.pbio.1000554
Park, Chad K. ; Joshi, Hemant K. ; Agrawal, Alka ; Ghare, M. Imran ; Little, Elizabeth J. ; Dunten, Pete W. ; Bitinaite, Jurate ; Horton, Nancy C. / Domain swapping in allosteric modulation of DNA specificity. In: PLoS Biology. 2010 ; Vol. 8, No. 12.
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