Dominant point mutation in a tetraspanin gene associated with field-evolved resistance of cotton bollworm to transgenic Bt cotton

Lin Jin, Jing Wang, Fang Guan, Jianpeng Zhang, Shan Yu, Shaoyan Liu, Yuanyuan Xue, Lingli Li, Shuwen Wu, Xingliang Wang, Yihua Yang, Heba Abdelgaffar, Juan Luis Jurat-Fuentes, Bruce E Tabashnik, Yidong Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Extensive planting of crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) has suppressed some major pests, reduced insecticide sprays, enhanced pest control by natural enemies, and increased grower profits. However, rapid evolution of resistance in pests is reducing these benefits. Better understanding of the genetic basis of resistance to Bt crops is urgently needed to monitor, delay, and counter pest resistance. We discovered that a point mutation in a previously unknown tetraspanin gene in the cotton bollworm (Helicoverpa armigera), a devastating global pest, confers dominant resistance to Cry1Ac, the sole Bt protein produced by transgenic cotton planted in China. We found the mutation using a genome-wide association study, followed by fine-scale genetic mapping and DNA sequence comparisons between resistant and susceptible strains. CRISPR/Cas9 knockout of the tetraspanin gene restored susceptibility to a resistant strain, whereas inserting the mutation conferred 125-fold resistance in a susceptible strain. DNA screening of moths captured from 23 field sites in six provinces of northern China revealed a 100-fold increase in the frequency of this mutation, from 0.001 in 2006 to 0.10 in 2016. The correspondence between the observed trajectory of the mutation and the trajectory predicted from simulation modeling shows that the dominance of the mutation accelerated adaptation. Proactive identification and tracking of the tetraspanin mutation demonstrate the potential for genomic analysis, gene editing, and molecular monitoring to improve management of resistance.

Original languageEnglish (US)
Pages (from-to)11760-11765
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number46
DOIs
StatePublished - Nov 13 2018

Fingerprint

Bacillus thuringiensis
Point Mutation
Mutation
Genes
China
Clustered Regularly Interspaced Short Palindromic Repeats
Pest Control
Gene Knockout Techniques
Moths
Genome-Wide Association Study
Mutation Rate
Insecticides
Bacteria
DNA
Proteins

Keywords

  • dominance
  • evolution
  • genetically modified
  • resistance management
  • sustainability

ASJC Scopus subject areas

  • General

Cite this

Dominant point mutation in a tetraspanin gene associated with field-evolved resistance of cotton bollworm to transgenic Bt cotton. / Jin, Lin; Wang, Jing; Guan, Fang; Zhang, Jianpeng; Yu, Shan; Liu, Shaoyan; Xue, Yuanyuan; Li, Lingli; Wu, Shuwen; Wang, Xingliang; Yang, Yihua; Abdelgaffar, Heba; Jurat-Fuentes, Juan Luis; Tabashnik, Bruce E; Wu, Yidong.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 46, 13.11.2018, p. 11760-11765.

Research output: Contribution to journalArticle

Jin, L, Wang, J, Guan, F, Zhang, J, Yu, S, Liu, S, Xue, Y, Li, L, Wu, S, Wang, X, Yang, Y, Abdelgaffar, H, Jurat-Fuentes, JL, Tabashnik, BE & Wu, Y 2018, 'Dominant point mutation in a tetraspanin gene associated with field-evolved resistance of cotton bollworm to transgenic Bt cotton', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 46, pp. 11760-11765. https://doi.org/10.1073/pnas.1812138115
Jin, Lin ; Wang, Jing ; Guan, Fang ; Zhang, Jianpeng ; Yu, Shan ; Liu, Shaoyan ; Xue, Yuanyuan ; Li, Lingli ; Wu, Shuwen ; Wang, Xingliang ; Yang, Yihua ; Abdelgaffar, Heba ; Jurat-Fuentes, Juan Luis ; Tabashnik, Bruce E ; Wu, Yidong. / Dominant point mutation in a tetraspanin gene associated with field-evolved resistance of cotton bollworm to transgenic Bt cotton. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 46. pp. 11760-11765.
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