Dominant resistance to Bt cotton and minor cross-resistance to Bt toxin Cry2Ab in cotton bollworm from China

Lin Jin, Yiyun Wei, Lei Zhang, Yihua Yang, Bruce E Tabashnik, Yidong Wu

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Evolution of resistance by insect pests threatens the long-term benefits of transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt). Previous work has detected increases in the frequency of resistance to Bt toxin Cry1Ac in populations of cotton bollworm, Helicoverpa armigera, from northern China where Bt cotton producing Cry1Ac has been grown extensively for more than a decade. Confirming that trend, we report evidence from 2011 showing that the percentage of individuals resistant to a diagnostic concentration of Cry1Ac was significantly higher in two populations from different provinces of northern China (1.4% and 2.3%) compared with previously tested susceptible field populations (0%). We isolated two resistant strains: one from each of the two field-selected populations. Relative to a susceptible strain, the two strains had 460- and 1200-fold resistance to Cry1Ac, respectively. Both strains had dominant resistance to a diagnostic concentration of Cry1Ac in diet and to Bt cotton leaves containing Cry1Ac. Both strains had low, but significant cross-resistance to Cry2Ab (4.2- and 5.9-fold), which is used widely as the second toxin in two-toxin Bt cotton. Compared with resistance in other strains of H. armigera, the resistance in the two strains characterized here may be especially difficult to suppress.

Original languageEnglish (US)
Pages (from-to)1222-1235
Number of pages14
JournalEvolutionary Applications
Volume6
Issue number8
DOIs
StatePublished - Dec 2013

Fingerprint

Bacillus thuringiensis
cross resistance
Helicoverpa armigera
toxin
cotton
China
toxins
Population
fold
insecticidal proteins
Insects
insect
diet
crop
protein
Diet
insect pests
genetically modified organisms
Proteins
crops

Keywords

  • Dominant resistance
  • Resistance evolution
  • Resistance management

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Dominant resistance to Bt cotton and minor cross-resistance to Bt toxin Cry2Ab in cotton bollworm from China. / Jin, Lin; Wei, Yiyun; Zhang, Lei; Yang, Yihua; Tabashnik, Bruce E; Wu, Yidong.

In: Evolutionary Applications, Vol. 6, No. 8, 12.2013, p. 1222-1235.

Research output: Contribution to journalArticle

Jin, Lin ; Wei, Yiyun ; Zhang, Lei ; Yang, Yihua ; Tabashnik, Bruce E ; Wu, Yidong. / Dominant resistance to Bt cotton and minor cross-resistance to Bt toxin Cry2Ab in cotton bollworm from China. In: Evolutionary Applications. 2013 ; Vol. 6, No. 8. pp. 1222-1235.
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