Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation

Mahmood S. Choudhery, Michael Badowski, Angela Muise, John Pierce, David T. Harris

Research output: Contribution to journalArticle

213 Scopus citations

Abstract

Background: Human adipose tissue is an ideal autologous source of mesenchymal stem cells (MSCs) for various regenerative medicine and tissue engineering strategies. Aged patients are one of the primary target populations for many promising applications. It has long been known that advanced age is negatively correlated with an organism's reparative and regenerative potential, but little and conflicting information is available about the effects of age on the quality of human adipose tissue derived MSCs (hAT-MSCs).Methods: To study the influence of age, the expansion and in vitro differentiation potential of hAT-MSCs from young (<30 years), adult (35-50 years) and aged (>60 years) individuals were investigated. MSCs were characterized for expression of the genes p16INK4a and p21 along with measurements of population doublings (PD), superoxide dismutase (SOD) activity, cellular senescence and differentiation potential.Results: Aged MSCs displayed senescent features when compared with cells isolated from young donors, concomitant with reduced viability and proliferation. These features were also associated with significantly reduced differentiation potential in aged MSCs compared to young MSCs.Conclusions: In conclusion, advancing age negatively impacts stem cell function and such age related alterations may be detrimental for successful stem cell therapies.

Original languageEnglish (US)
Article number8
JournalJournal of Translational Medicine
Volume12
Issue number1
DOIs
StatePublished - Jan 7 2014

Keywords

  • Adipose tissue
  • Donor age
  • Growth kinetics
  • In vitro differentiation potential
  • Mesenchymal stem cells
  • Regenerative potential

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation'. Together they form a unique fingerprint.

  • Cite this