Dose-dependent transcriptome changes by metal ores on a human acute lymphoblastic leukemia cell line

Nina N. Sun, Cynthia D. Fastje, Simon S. Wong, Paul R. Sheppard, Stephanie J. Macdonald, Gary Ridenour, Juanita D. Hyde, Mark L. Witten

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The increased morbidity of childhood leukemia in Fallon, Nevada and Sierra Vista, Arizona has prompted great health concern. The main characteristic that these two towns share is the environmental pollution attributed to metal ore from abandoned mining operations. Consequently, we have investigated the transcriptome effects of metal ores from these endemic areas using a human T-cell acute lymphoblastic leukemia cell line (T-ALL). Metal ore from Fallon significantly increased cell growth after 24, 48 and 72 h of incubation at 1.5 mg/mL concentration, as measured by trypan-blue. Sierra Vista ore significantly increased cell growth with 0.15 and 1.5 mg/mL following 72 h of incubation. From human cDNA microarray, results indicate that in total, eight genes, mostly metallothionein (MT) genes, were up-regulated and 10 genes were down-regulated following treatment of the T-ALL cells with 0.15 and 1.5 mg/mL of metal ores at 72 h, in comparison with untreated cells. Twenty-eight metals of both ores were quantified and their presence may be associated with the cell growth rate and dose-dependent activation of transcriptomes in immature T-cells.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalToxicology and Industrial Health
Volume19
Issue number10
DOIs
StatePublished - Aug 2003

Keywords

  • gene microarray
  • leukemia
  • metallothionein
  • metals
  • transcriptomes
  • tungsten

ASJC Scopus subject areas

  • Toxicology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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    Sun, N. N., Fastje, C. D., Wong, S. S., Sheppard, P. R., Macdonald, S. J., Ridenour, G., Hyde, J. D., & Witten, M. L. (2003). Dose-dependent transcriptome changes by metal ores on a human acute lymphoblastic leukemia cell line. Toxicology and Industrial Health, 19(10), 157-163. https://doi.org/10.1191/0748233703th185oa