Double-outlet right ventricle and overriding tricuspid valve reflect disturbances of looping, myocardialization, endocardial cushion differentiation, and apoptosis in TGF-β2-knockout mice

Ulrike Bartram, Daniël G M Molin, Lambertus J. Wisse, Azhar Mohamad, L. Philip Sanford, Thomas C Doetschman, Christian P. Speer, Robert E. Poelmann, Adriana C. Gittenberger-de Groot

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

Background - Transforming growth factor-β2 (TGF-β2) is a member of a family of growth factors with the potential to modify multiple processes. Mice deficient in the TGF-β2 gene die around birth and show a variety of defects of different organs, including the heart. Methods and Results - We studied the hearts of TGF-β2-null mouse embryos from 11.5 to 18.5 days of gestation to analyze the types of defects and determine which processes of cardiac morphogenesis are affected by the absence of TGF-β2. Analysis of serial sections revealed malformations of the outflow tract (typically a double-outlet right ventricle) in 87.5%. There was 1 case of common arterial trunk. Abnormal thickening of the semilunar valves was seen in 4.2%. Associated malformations of the atrioventricular (AV) canal were found in 62.5% and were composed of perimembranous inlet ventricular septal defects (37.5%), AV valve thickening (33.3%), overriding tricuspid valve (25.0%), and complete AV septal defects (4.2%). Anomalies of the aorta and its branches were seen in 33.3%. Immunohistochemical staining showed failure of myocardialization of the mesenchyme of the atrial septum and the ventricular outflow tract as well as deficient valve differentiation. Morphometry documented this to be associated with absence of the normal decrease of total endocardial cushion volume in the older stages. Apoptosis in TGF-β2-knockout mice was increased, although regional distribution was normal. Conclusions - TGF-β2-knockout mice exhibited characteristic cardiovascular anomalies comparable to malformations seen in the human population.

Original languageEnglish (US)
Pages (from-to)2745-2752
Number of pages8
JournalCirculation
Volume103
Issue number22
StatePublished - Jun 5 2001
Externally publishedYes

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Endocardial Cushions
Double Outlet Right Ventricle
Tricuspid Valve
Transforming Growth Factors
Knockout Mice
Apoptosis
Atrial Septum
Ventricular Heart Septal Defects
Normal Distribution
Mesoderm
Morphogenesis
Aorta
Intercellular Signaling Peptides and Proteins
Embryonic Structures
Parturition
Staining and Labeling
Pregnancy

Keywords

  • Genes
  • Growth substances
  • Heart defects, congenital
  • Morphogenesis

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Double-outlet right ventricle and overriding tricuspid valve reflect disturbances of looping, myocardialization, endocardial cushion differentiation, and apoptosis in TGF-β2-knockout mice. / Bartram, Ulrike; Molin, Daniël G M; Wisse, Lambertus J.; Mohamad, Azhar; Sanford, L. Philip; Doetschman, Thomas C; Speer, Christian P.; Poelmann, Robert E.; Gittenberger-de Groot, Adriana C.

In: Circulation, Vol. 103, No. 22, 05.06.2001, p. 2745-2752.

Research output: Contribution to journalArticle

Bartram, U, Molin, DGM, Wisse, LJ, Mohamad, A, Sanford, LP, Doetschman, TC, Speer, CP, Poelmann, RE & Gittenberger-de Groot, AC 2001, 'Double-outlet right ventricle and overriding tricuspid valve reflect disturbances of looping, myocardialization, endocardial cushion differentiation, and apoptosis in TGF-β2-knockout mice', Circulation, vol. 103, no. 22, pp. 2745-2752.
Bartram, Ulrike ; Molin, Daniël G M ; Wisse, Lambertus J. ; Mohamad, Azhar ; Sanford, L. Philip ; Doetschman, Thomas C ; Speer, Christian P. ; Poelmann, Robert E. ; Gittenberger-de Groot, Adriana C. / Double-outlet right ventricle and overriding tricuspid valve reflect disturbances of looping, myocardialization, endocardial cushion differentiation, and apoptosis in TGF-β2-knockout mice. In: Circulation. 2001 ; Vol. 103, No. 22. pp. 2745-2752.
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abstract = "Background - Transforming growth factor-β2 (TGF-β2) is a member of a family of growth factors with the potential to modify multiple processes. Mice deficient in the TGF-β2 gene die around birth and show a variety of defects of different organs, including the heart. Methods and Results - We studied the hearts of TGF-β2-null mouse embryos from 11.5 to 18.5 days of gestation to analyze the types of defects and determine which processes of cardiac morphogenesis are affected by the absence of TGF-β2. Analysis of serial sections revealed malformations of the outflow tract (typically a double-outlet right ventricle) in 87.5{\%}. There was 1 case of common arterial trunk. Abnormal thickening of the semilunar valves was seen in 4.2{\%}. Associated malformations of the atrioventricular (AV) canal were found in 62.5{\%} and were composed of perimembranous inlet ventricular septal defects (37.5{\%}), AV valve thickening (33.3{\%}), overriding tricuspid valve (25.0{\%}), and complete AV septal defects (4.2{\%}). Anomalies of the aorta and its branches were seen in 33.3{\%}. Immunohistochemical staining showed failure of myocardialization of the mesenchyme of the atrial septum and the ventricular outflow tract as well as deficient valve differentiation. Morphometry documented this to be associated with absence of the normal decrease of total endocardial cushion volume in the older stages. Apoptosis in TGF-β2-knockout mice was increased, although regional distribution was normal. Conclusions - TGF-β2-knockout mice exhibited characteristic cardiovascular anomalies comparable to malformations seen in the human population.",
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T1 - Double-outlet right ventricle and overriding tricuspid valve reflect disturbances of looping, myocardialization, endocardial cushion differentiation, and apoptosis in TGF-β2-knockout mice

AU - Bartram, Ulrike

AU - Molin, Daniël G M

AU - Wisse, Lambertus J.

AU - Mohamad, Azhar

AU - Sanford, L. Philip

AU - Doetschman, Thomas C

AU - Speer, Christian P.

AU - Poelmann, Robert E.

AU - Gittenberger-de Groot, Adriana C.

PY - 2001/6/5

Y1 - 2001/6/5

N2 - Background - Transforming growth factor-β2 (TGF-β2) is a member of a family of growth factors with the potential to modify multiple processes. Mice deficient in the TGF-β2 gene die around birth and show a variety of defects of different organs, including the heart. Methods and Results - We studied the hearts of TGF-β2-null mouse embryos from 11.5 to 18.5 days of gestation to analyze the types of defects and determine which processes of cardiac morphogenesis are affected by the absence of TGF-β2. Analysis of serial sections revealed malformations of the outflow tract (typically a double-outlet right ventricle) in 87.5%. There was 1 case of common arterial trunk. Abnormal thickening of the semilunar valves was seen in 4.2%. Associated malformations of the atrioventricular (AV) canal were found in 62.5% and were composed of perimembranous inlet ventricular septal defects (37.5%), AV valve thickening (33.3%), overriding tricuspid valve (25.0%), and complete AV septal defects (4.2%). Anomalies of the aorta and its branches were seen in 33.3%. Immunohistochemical staining showed failure of myocardialization of the mesenchyme of the atrial septum and the ventricular outflow tract as well as deficient valve differentiation. Morphometry documented this to be associated with absence of the normal decrease of total endocardial cushion volume in the older stages. Apoptosis in TGF-β2-knockout mice was increased, although regional distribution was normal. Conclusions - TGF-β2-knockout mice exhibited characteristic cardiovascular anomalies comparable to malformations seen in the human population.

AB - Background - Transforming growth factor-β2 (TGF-β2) is a member of a family of growth factors with the potential to modify multiple processes. Mice deficient in the TGF-β2 gene die around birth and show a variety of defects of different organs, including the heart. Methods and Results - We studied the hearts of TGF-β2-null mouse embryos from 11.5 to 18.5 days of gestation to analyze the types of defects and determine which processes of cardiac morphogenesis are affected by the absence of TGF-β2. Analysis of serial sections revealed malformations of the outflow tract (typically a double-outlet right ventricle) in 87.5%. There was 1 case of common arterial trunk. Abnormal thickening of the semilunar valves was seen in 4.2%. Associated malformations of the atrioventricular (AV) canal were found in 62.5% and were composed of perimembranous inlet ventricular septal defects (37.5%), AV valve thickening (33.3%), overriding tricuspid valve (25.0%), and complete AV septal defects (4.2%). Anomalies of the aorta and its branches were seen in 33.3%. Immunohistochemical staining showed failure of myocardialization of the mesenchyme of the atrial septum and the ventricular outflow tract as well as deficient valve differentiation. Morphometry documented this to be associated with absence of the normal decrease of total endocardial cushion volume in the older stages. Apoptosis in TGF-β2-knockout mice was increased, although regional distribution was normal. Conclusions - TGF-β2-knockout mice exhibited characteristic cardiovascular anomalies comparable to malformations seen in the human population.

KW - Genes

KW - Growth substances

KW - Heart defects, congenital

KW - Morphogenesis

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