Abstract
We have developed a double-stranded locked nucleic acid (dsLNA) probe for monitoring the intracellular gene expression toward the investigation of the role of mechanical injury in cancer metastasis. Specifically, the dsLNA probe is shown to allow real-time detection of intracellular mRNA in living cells. Using the dsLNA probes, the spatiotemporal gene expressions of cancer cells after mechanical injury are monitored for days. We show that the release of contact inhibition is sufficient to induce the migratory behavior of cancer cells while mechanical injury triggers the cellular antioxidant response in a region ~100 μm away from the wound.
| Original language | English (US) |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 109-111 |
| Number of pages | 3 |
| State | Published - Dec 1 2011 |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: Oct 2 2011 → Oct 6 2011 |
Publication series
| Name | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Volume | 1 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Country | United States |
| City | Seattle, WA |
| Period | 10/2/11 → 10/6/11 |
Keywords
- DsLNA probe
- HO-1 mRNA expression
- MDA-MB-231 cancer cells
- Wound healing assay
ASJC Scopus subject areas
- Control and Systems Engineering
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Cite this
Riahi, R., & Wong, P. K. (2011). Double-stranded locked nucleic acid probes for revealing intracellular gene expression dynamics of cancer cells near mechanical wounds. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 109-111). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 1).