Doubling genome size without polyploidization: Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice

Benoit Piegu, Romain Guyot, Nathalie Picault, Anne Roulin, Abhijit Saniyal, Hyeran Kim, Kristi Collura, Darshan S. Brar, Scott Jackson, Rod A. Wing, Olivier Panaud

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370 Scopus citations

Abstract

Retrotransposons are the main components of eukaryotic genomes, representing up to 80% of some large plant genomes. These mobile elements transpose via a "copy and paste" mechanism, thus increasing their copy number while active. Their accumulation is now accepted as the main factor of genome size increase in higher eukaryotes, besides polyploidy. However, the dynamics of this process are poorly understood. In this study, we show that Oryza australiensis, a wild relative of the Asian cultivated rice O. sativa, has undergone recent bursts of three LTR-retrotransposon families. This genome has accumulated more than 90,000 retrotransposon copies during the last three million years, leading to a rapid twofold increase of its size. In addition, phenetic analyses of these retrotransposons clearly confirm that the genomic bursts occurred posterior to the radiation of the species. This provides direct evidence of retrotransposon-mediated variation of genome size within a plant genus.

Original languageEnglish (US)
Pages (from-to)1262-1269
Number of pages8
JournalGenome Research
Volume16
Issue number10
DOIs
StatePublished - Oct 10 2006

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ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Piegu, B., Guyot, R., Picault, N., Roulin, A., Saniyal, A., Kim, H., Collura, K., Brar, D. S., Jackson, S., Wing, R. A., & Panaud, O. (2006). Doubling genome size without polyploidization: Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice. Genome Research, 16(10), 1262-1269. https://doi.org/10.1101/gr.5290206