TY - JOUR
T1 - Doubling genome size without polyploidization
T2 - Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice
AU - Piegu, Benoit
AU - Guyot, Romain
AU - Picault, Nathalie
AU - Roulin, Anne
AU - Saniyal, Abhijit
AU - Kim, Hyeran
AU - Collura, Kristi
AU - Brar, Darshan S.
AU - Jackson, Scott
AU - Wing, Rod A.
AU - Panaud, Olivier
PY - 2006
Y1 - 2006
N2 - Retrotransposons are the main components of eukaryotic genomes, representing up to 80% of some large plant genomes. These mobile elements transpose via a "copy and paste" mechanism, thus increasing their copy number while active. Their accumulation is now accepted as the main factor of genome size increase in higher eukaryotes, besides polyploidy. However, the dynamics of this process are poorly understood. In this study, we show that Oryza australiensis, a wild relative of the Asian cultivated rice O. sativa, has undergone recent bursts of three LTR-retrotransposon families. This genome has accumulated more than 90,000 retrotransposon copies during the last three million years, leading to a rapid twofold increase of its size. In addition, phenetic analyses of these retrotransposons clearly confirm that the genomic bursts occurred posterior to the radiation of the species. This provides direct evidence of retrotransposon-mediated variation of genome size within a plant genus.
AB - Retrotransposons are the main components of eukaryotic genomes, representing up to 80% of some large plant genomes. These mobile elements transpose via a "copy and paste" mechanism, thus increasing their copy number while active. Their accumulation is now accepted as the main factor of genome size increase in higher eukaryotes, besides polyploidy. However, the dynamics of this process are poorly understood. In this study, we show that Oryza australiensis, a wild relative of the Asian cultivated rice O. sativa, has undergone recent bursts of three LTR-retrotransposon families. This genome has accumulated more than 90,000 retrotransposon copies during the last three million years, leading to a rapid twofold increase of its size. In addition, phenetic analyses of these retrotransposons clearly confirm that the genomic bursts occurred posterior to the radiation of the species. This provides direct evidence of retrotransposon-mediated variation of genome size within a plant genus.
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U2 - 10.1101/gr.5290206
DO - 10.1101/gr.5290206
M3 - Article
C2 - 16963705
AN - SCOPUS:33749392949
VL - 16
SP - 1262
EP - 1269
JO - PCR Methods and Applications
JF - PCR Methods and Applications
SN - 1088-9051
IS - 10
ER -