Downstream trends of in vitro bioassay responses in a wastewater effluent-dominated river

Kevin D. Daniels, Darcy VanDervort, Shimin Wu, Frederic D.L. Leusch, Jason P. van de Merwe, Ai Jia, Shane A Snyder

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Surface waters are becoming increasingly influenced by wastewater effluents due to drought conditions, growing populations, and urbanization. These effluents contain mixtures of trace organic compounds (TOrCs), including bioactive constituents, which are not fully attenuated by conventional wastewater treatment systems. This study investigated the occurrence of glucocorticoid receptor (GR), aryl hydrocarbon receptor (AhR), and estrogen receptor (ER) activity, as well as the overall toxicity to bacteria (BLT-Screen), in the effluent of two wastewater reclamation facilities (WRF) and downstream of the Lower Santa Cruz River, Pima County, Arizona USA, which is dominated by the WRF effluents. The GR, AhR, and ER activities and toxicity to bacteria were determined by in vitro bioassays during four seasons. Bioassay results showed the highest activities at the wastewater outfalls, with activities decreasing downstream of the river. Biological equivalent concentrations ranged from 9 to 170 ng/L dexamethasone-equivalents (DexEQ), 0.1–0.8 ng/L 2,3,7,8-tetrachlorodibenzo-p-dioxin-equivalents (TCDDEQ), and <0.005–0.8 ng/L estradiol equivalents (EEQ) for GR-, AhR- and ER-mediated activity, respectively. This level of biological activity at times exceeded the relevant effects-based trigger value for environmental effects, indicating a potential risk to the receiving environment. Toxicity to bacteria was low at all sites, well below the trigger value of 1.0 TUIC20, which represents an undiluted water sample causing 20% toxicity in the assay. The potential inducing glucocorticoid agonists were further analysed by liquid chromatography coupled to tandem mass spectrometry. Analytical results reveal triamcinolone acetonide as the most abundant glucocorticoid with concentrations up to 38 ng/L. Similar results for DexEQ concentrations calculated from both chemical and bioassay data indicate a successful mass balance for glucocorticoids. This mass balance illustrated lower DexEQ during summer months, which could be due to an increased attenuation from photodegradation.

Original languageEnglish (US)
Pages (from-to)182-192
Number of pages11
JournalChemosphere
Volume212
DOIs
StatePublished - Dec 1 2018

Fingerprint

Bioassay
Waste Water
Rivers
Aryl Hydrocarbon Receptors
Biological Assay
Toxicity
bioassay
Effluents
Glucocorticoid Receptors
Wastewater
Estrogen Receptors
Dexamethasone
effluent
Glucocorticoids
Wastewater reclamation
toxicity
wastewater
Bacteria
hydrocarbon
river

Keywords

  • AhR
  • BLT-Screen
  • Estrogenic
  • Glucocorticoid
  • In vitro bioassay
  • Seasonal variation

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Daniels, K. D., VanDervort, D., Wu, S., Leusch, F. D. L., van de Merwe, J. P., Jia, A., & Snyder, S. A. (2018). Downstream trends of in vitro bioassay responses in a wastewater effluent-dominated river. Chemosphere, 212, 182-192. https://doi.org/10.1016/j.chemosphere.2018.07.190

Downstream trends of in vitro bioassay responses in a wastewater effluent-dominated river. / Daniels, Kevin D.; VanDervort, Darcy; Wu, Shimin; Leusch, Frederic D.L.; van de Merwe, Jason P.; Jia, Ai; Snyder, Shane A.

In: Chemosphere, Vol. 212, 01.12.2018, p. 182-192.

Research output: Contribution to journalArticle

Daniels, KD, VanDervort, D, Wu, S, Leusch, FDL, van de Merwe, JP, Jia, A & Snyder, SA 2018, 'Downstream trends of in vitro bioassay responses in a wastewater effluent-dominated river', Chemosphere, vol. 212, pp. 182-192. https://doi.org/10.1016/j.chemosphere.2018.07.190
Daniels KD, VanDervort D, Wu S, Leusch FDL, van de Merwe JP, Jia A et al. Downstream trends of in vitro bioassay responses in a wastewater effluent-dominated river. Chemosphere. 2018 Dec 1;212:182-192. https://doi.org/10.1016/j.chemosphere.2018.07.190
Daniels, Kevin D. ; VanDervort, Darcy ; Wu, Shimin ; Leusch, Frederic D.L. ; van de Merwe, Jason P. ; Jia, Ai ; Snyder, Shane A. / Downstream trends of in vitro bioassay responses in a wastewater effluent-dominated river. In: Chemosphere. 2018 ; Vol. 212. pp. 182-192.
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