Drosophila melanogaster CYP6A8, an insect P450 that catalyzes lauric acid (ω-1)-hydroxylation

Christian Helvig, Nathalie Tijet, René Feyereisen, F. Ann Walker, Linda L. Restifo

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Only a handful of P450 genes have been functionally characterized from the approximately 90 recently identified in the genome of Drosophila melanogaster. Cyp6a8 encodes a 506-amino acid protein with 53.6% amino acid identity with CYP6A2. CYP6A2 has been shown to catalyze the metabolism of several insecticides including aldrin and heptachlor. CYP6A8 is expressed at many developmental stages as well as in adult life. CYP6A8 was produced in Saccharomyces cerevisiae and enzymatically characterized after catalytic activity was reconstituted with D. melanogaster P450 reductase and NADPH. Although several saturated or non-saturated fatty acids were not metabolized by CYP6A8, lauric acid (C12:0), a short-chain unsaturated fatty acid, was oxidized by CYP6A8 to produce 11-hydroxylauric acid with an apparent V max of 25 nmol/min/nmol P450. This is the first report showing that a member of the CYP6 family catalyzes the hydroxylation of lauric acid. Our data open new prospects for the CYP6 P450 enzymes, which could be involved in important physiological functions through fatty acid metabolism.

Original languageEnglish (US)
Pages (from-to)1495-1502
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume325
Issue number4
DOIs
StatePublished - Dec 24 2004

Keywords

  • Cytochrome P450
  • Development
  • Drosophila melanogaster
  • Fatty acids
  • HPLC
  • Hydroxylation
  • Insecticides
  • Lauric acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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