Drosophila PLUTONIUM protein is a specialized cell cycle regulator required at the onset of embryogenesis

Lisa K Elfring, J. Myles Axton, Douglas D. Fenger, Andrea W. Page, Janet L. Carminati, Terry L. Orr-Weaver

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Unfertilized eggs and fertilized embryos from Drosophila mothers mutant for the plutonium (plu) gene contain giant polyploid nuclei resulting from unregulated S-phase. The PLU protein, a 19-kDa ankyrin repeat protein, is present in oocytes and early embryos but is not detectable after the completion of the initial rapid S-M cycles of the embryo. The persistence of the protein during the early embryonic divisions is consistent with a direct role in linking S-phase and M-phase. When ectopically expressed in the eye disc, PLU did not perturb the cell cycle, suggesting that PLU regulates S- phase only in early embryonic development. The pan gu (png) and giant nuclei (gnu) genes also affect the S-phase in the unfertilized egg and early embryo. We show that functional png is needed for the presence of PLU protein. By analyzing png mutations of differing severity, we find that the extent of the png mutant phenotype inversely reflects the level of PLU protein. Our data suggest that PLU protein is required at the time of egg activation and the completion of meiosis.

Original languageEnglish (US)
Pages (from-to)583-593
Number of pages11
JournalMolecular Biology of the Cell
Volume8
Issue number4
StatePublished - Apr 1997
Externally publishedYes

Fingerprint

Drosophila Proteins
Embryonic Development
Cell Cycle
S Phase
Embryonic Structures
Ovum
Proteins
Ankyrin Repeat
Plutonium
Polyploidy
Meiosis
Cell Division
Genes
Drosophila
Oocytes
Phenotype
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Elfring, L. K., Axton, J. M., Fenger, D. D., Page, A. W., Carminati, J. L., & Orr-Weaver, T. L. (1997). Drosophila PLUTONIUM protein is a specialized cell cycle regulator required at the onset of embryogenesis. Molecular Biology of the Cell, 8(4), 583-593.

Drosophila PLUTONIUM protein is a specialized cell cycle regulator required at the onset of embryogenesis. / Elfring, Lisa K; Axton, J. Myles; Fenger, Douglas D.; Page, Andrea W.; Carminati, Janet L.; Orr-Weaver, Terry L.

In: Molecular Biology of the Cell, Vol. 8, No. 4, 04.1997, p. 583-593.

Research output: Contribution to journalArticle

Elfring, LK, Axton, JM, Fenger, DD, Page, AW, Carminati, JL & Orr-Weaver, TL 1997, 'Drosophila PLUTONIUM protein is a specialized cell cycle regulator required at the onset of embryogenesis', Molecular Biology of the Cell, vol. 8, no. 4, pp. 583-593.
Elfring, Lisa K ; Axton, J. Myles ; Fenger, Douglas D. ; Page, Andrea W. ; Carminati, Janet L. ; Orr-Weaver, Terry L. / Drosophila PLUTONIUM protein is a specialized cell cycle regulator required at the onset of embryogenesis. In: Molecular Biology of the Cell. 1997 ; Vol. 8, No. 4. pp. 583-593.
@article{90872bf61933439cb82d8d9f9efc5b4b,
title = "Drosophila PLUTONIUM protein is a specialized cell cycle regulator required at the onset of embryogenesis",
abstract = "Unfertilized eggs and fertilized embryos from Drosophila mothers mutant for the plutonium (plu) gene contain giant polyploid nuclei resulting from unregulated S-phase. The PLU protein, a 19-kDa ankyrin repeat protein, is present in oocytes and early embryos but is not detectable after the completion of the initial rapid S-M cycles of the embryo. The persistence of the protein during the early embryonic divisions is consistent with a direct role in linking S-phase and M-phase. When ectopically expressed in the eye disc, PLU did not perturb the cell cycle, suggesting that PLU regulates S- phase only in early embryonic development. The pan gu (png) and giant nuclei (gnu) genes also affect the S-phase in the unfertilized egg and early embryo. We show that functional png is needed for the presence of PLU protein. By analyzing png mutations of differing severity, we find that the extent of the png mutant phenotype inversely reflects the level of PLU protein. Our data suggest that PLU protein is required at the time of egg activation and the completion of meiosis.",
author = "Elfring, {Lisa K} and Axton, {J. Myles} and Fenger, {Douglas D.} and Page, {Andrea W.} and Carminati, {Janet L.} and Orr-Weaver, {Terry L.}",
year = "1997",
month = "4",
language = "English (US)",
volume = "8",
pages = "583--593",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "4",

}

TY - JOUR

T1 - Drosophila PLUTONIUM protein is a specialized cell cycle regulator required at the onset of embryogenesis

AU - Elfring, Lisa K

AU - Axton, J. Myles

AU - Fenger, Douglas D.

AU - Page, Andrea W.

AU - Carminati, Janet L.

AU - Orr-Weaver, Terry L.

PY - 1997/4

Y1 - 1997/4

N2 - Unfertilized eggs and fertilized embryos from Drosophila mothers mutant for the plutonium (plu) gene contain giant polyploid nuclei resulting from unregulated S-phase. The PLU protein, a 19-kDa ankyrin repeat protein, is present in oocytes and early embryos but is not detectable after the completion of the initial rapid S-M cycles of the embryo. The persistence of the protein during the early embryonic divisions is consistent with a direct role in linking S-phase and M-phase. When ectopically expressed in the eye disc, PLU did not perturb the cell cycle, suggesting that PLU regulates S- phase only in early embryonic development. The pan gu (png) and giant nuclei (gnu) genes also affect the S-phase in the unfertilized egg and early embryo. We show that functional png is needed for the presence of PLU protein. By analyzing png mutations of differing severity, we find that the extent of the png mutant phenotype inversely reflects the level of PLU protein. Our data suggest that PLU protein is required at the time of egg activation and the completion of meiosis.

AB - Unfertilized eggs and fertilized embryos from Drosophila mothers mutant for the plutonium (plu) gene contain giant polyploid nuclei resulting from unregulated S-phase. The PLU protein, a 19-kDa ankyrin repeat protein, is present in oocytes and early embryos but is not detectable after the completion of the initial rapid S-M cycles of the embryo. The persistence of the protein during the early embryonic divisions is consistent with a direct role in linking S-phase and M-phase. When ectopically expressed in the eye disc, PLU did not perturb the cell cycle, suggesting that PLU regulates S- phase only in early embryonic development. The pan gu (png) and giant nuclei (gnu) genes also affect the S-phase in the unfertilized egg and early embryo. We show that functional png is needed for the presence of PLU protein. By analyzing png mutations of differing severity, we find that the extent of the png mutant phenotype inversely reflects the level of PLU protein. Our data suggest that PLU protein is required at the time of egg activation and the completion of meiosis.

UR - http://www.scopus.com/inward/record.url?scp=0030960799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030960799&partnerID=8YFLogxK

M3 - Article

C2 - 9247640

AN - SCOPUS:0030960799

VL - 8

SP - 583

EP - 593

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 4

ER -