Dual modality instrument for simultaneous optical coherence tomography imaging and fluorescence spectroscopy

Jennifer K Barton, Francisco Guzman, Alexandre Tumlinson

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We develop a dual-modality device that combines the anatomical imaging capabilities of optical coherence tomography (OCT) with the functional capabilities of laser-induced fluorescence (LIF) spectroscopy. OCT provides cross-sectional images of tissue structure to a depth of up to 2 mm with approximately 10-μm resolution. LIF spectroscopy provides histochemical information in the form of emission spectra from a given tissue location. The OCT subsystem utilizes a superluminescent diode with a center wavelength of 1300 nm, whereas a helium cadmium laser provides the LIF excitation source at wavelengths of 325 and 442 nm. Preliminary data are obtained on eight postmortem aorta samples, each 10 mm in length. OCT images and LIF spectra give complementary information from normal and atherosclerotic portions of aorta wall. OCT images show structures such as intima, media, internal elastic lamina, and fibrotic regions. Emission spectra ratios of 520/490 (325-nm excitation) and 595/635 (442-nm excitation) could be used to identify normal and plaque regions with 97 and 91% correct classification rates, respectively. With miniaturization of the delivery probe and improvements in system speed, this dual-modality device could provide a valuable tool for identification and characterization of atherosclerotic plaques.

Original languageEnglish (US)
Pages (from-to)618-623
Number of pages6
JournalJournal of Biomedical Optics
Volume9
Issue number3
DOIs
StatePublished - May 2004

Fingerprint

Optical tomography
Fluorescence Spectrometry
Optical Coherence Tomography
Fluorescence spectroscopy
tomography
laser induced fluorescence
Imaging techniques
Lasers
fluorescence
aorta
spectroscopy
Aorta
emission spectra
Fluorescence
Tissue
excitation
Miniaturization
Equipment and Supplies
Wavelength
Helium

Keywords

  • Aorta
  • Atherosclerosis
  • Laser-induced fluorescence
  • Multimodality
  • Plaque

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Dual modality instrument for simultaneous optical coherence tomography imaging and fluorescence spectroscopy. / Barton, Jennifer K; Guzman, Francisco; Tumlinson, Alexandre.

In: Journal of Biomedical Optics, Vol. 9, No. 3, 05.2004, p. 618-623.

Research output: Contribution to journalArticle

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