Dual mode of action of Bt proteins: Protoxin efficacy against resistant insects

Bruce E Tabashnik, Min Zhang, Jeffrey A. Fabrick, Yidong Wu, Meijing Gao, Fangneng Huang, Jizhen Wei, Jie Zhang, Alexander Yelich, Gopalan C. Unnithan, Alejandra Bravo, Mario Soberón, Yves Carriere, Xianchun Li

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Transgenic crops that produce Bacillus thuringiensis (Bt) proteins for pest control are grown extensively, but insect adaptation can reduce their effectiveness. Established mode of action models assert that Bt proteins Cry1Ab and Cry1Ac are produced as inactive protoxins that require conversion to a smaller activated form to exert toxicity. However, contrary to this widely accepted paradigm, we report evidence from seven resistant strains of three major crop pests showing that Cry1Ab and Cry1Ac protoxins were generally more potent than the corresponding activated toxins. Moreover, resistance was higher to activated toxins than protoxins in eight of nine cases evaluated in this study. These data and previously reported results support a new model in which protoxins and activated toxins kill insects via different pathways. Recognizing that protoxins can be more potent than activated toxins against resistant insects may help to enhance and sustain the efficacy of transgenic Bt crops.

Original languageEnglish (US)
Article number15107
JournalScientific Reports
Volume5
DOIs
StatePublished - Oct 12 2015

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Insects
Bacillus thuringiensis
Pest Control
Proteins
Bacillus thuringiensis protoxin
Bacillus Thuringiensis insecticidal crystal protein

ASJC Scopus subject areas

  • General

Cite this

Dual mode of action of Bt proteins : Protoxin efficacy against resistant insects. / Tabashnik, Bruce E; Zhang, Min; Fabrick, Jeffrey A.; Wu, Yidong; Gao, Meijing; Huang, Fangneng; Wei, Jizhen; Zhang, Jie; Yelich, Alexander; Unnithan, Gopalan C.; Bravo, Alejandra; Soberón, Mario; Carriere, Yves; Li, Xianchun.

In: Scientific Reports, Vol. 5, 15107, 12.10.2015.

Research output: Contribution to journalArticle

Tabashnik, BE, Zhang, M, Fabrick, JA, Wu, Y, Gao, M, Huang, F, Wei, J, Zhang, J, Yelich, A, Unnithan, GC, Bravo, A, Soberón, M, Carriere, Y & Li, X 2015, 'Dual mode of action of Bt proteins: Protoxin efficacy against resistant insects', Scientific Reports, vol. 5, 15107. https://doi.org/10.1038/srep15107
Tabashnik, Bruce E ; Zhang, Min ; Fabrick, Jeffrey A. ; Wu, Yidong ; Gao, Meijing ; Huang, Fangneng ; Wei, Jizhen ; Zhang, Jie ; Yelich, Alexander ; Unnithan, Gopalan C. ; Bravo, Alejandra ; Soberón, Mario ; Carriere, Yves ; Li, Xianchun. / Dual mode of action of Bt proteins : Protoxin efficacy against resistant insects. In: Scientific Reports. 2015 ; Vol. 5.
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