Dural afferents express acid-sensing ion channels: A role for decreased meningeal pH in migraine headache

Jin Yan, Rebecca M. Edelmayer, Xiaomei Wei, Milena De Felice, Frank Porreca, Gregory Dussor

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Migraine headache is one of the most common neurological disorders. The pathological conditions that directly initiate afferent pain signaling are poorly understood. In trigeminal neurons retrogradely labeled from the cranial meninges, we have recorded pH-evoked currents using whole-cell patch-clamp electrophysiology. Approximately 80% of dural-afferent neurons responded to a pH 6.0 application with a rapidly activating and rapidly desensitizing ASIC-like current that often exceeded 20 nA in amplitude. Inward currents were observed in response to a wide range of pH values and 30% of the neurons exhibited inward currents at pH 7.1. These currents led to action potentials in 53%, 30% and 7% of the dural afferents at pH 6.8, 6.9 and 7.0, respectively. Small decreases in extracellular pH were also able to generate sustained window currents and sustained membrane depolarizations. Amiloride, a non-specific blocker of ASIC channels, inhibited the peak currents evoked upon application of decreased pH while no inhibition was observed upon application of TRPV1 antagonists. The desensitization time constant of pH 6.0-evoked currents in the majority of dural afferents was less than 500 ms which is consistent with that reported for ASIC3 homomeric or heteromeric channels. Finally, application of pH 5.0 synthetic-interstitial fluid to the dura produced significant decreases in facial and hind-paw withdrawal threshold, an effect blocked by amiloride but not TRPV1 antagonists, suggesting that ASIC activation produces migraine-related behavior in vivo. These data provide a cellular mechanism by which decreased pH in the meninges following ischemic or inflammatory events directly excites afferent pain-sensing neurons potentially contributing to migraine headache.

Original languageEnglish (US)
Pages (from-to)106-113
Number of pages8
JournalPain
Volume152
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Acid Sensing Ion Channels
Migraine Disorders
Meninges
Amiloride
Neurons
Acid Sensing Ion Channel Blockers
Afferent Neurons
Pain
Electrophysiology
Extracellular Fluid
Nervous System Diseases
Action Potentials

Keywords

  • ASIC
  • Dural afferent
  • Headache
  • Meninges
  • Migraine
  • Pain

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology

Cite this

Dural afferents express acid-sensing ion channels : A role for decreased meningeal pH in migraine headache. / Yan, Jin; Edelmayer, Rebecca M.; Wei, Xiaomei; Felice, Milena De; Porreca, Frank; Dussor, Gregory.

In: Pain, Vol. 152, No. 1, 01.2011, p. 106-113.

Research output: Contribution to journalArticle

Yan, Jin ; Edelmayer, Rebecca M. ; Wei, Xiaomei ; Felice, Milena De ; Porreca, Frank ; Dussor, Gregory. / Dural afferents express acid-sensing ion channels : A role for decreased meningeal pH in migraine headache. In: Pain. 2011 ; Vol. 152, No. 1. pp. 106-113.
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