Dynamic expression of imprinted genes associates with maternally controlled nutrient allocation during maize endosperm development

Mingming Xin, Ruolin Yang, Guosheng Li, Hao Chen, John Laurie, Chuang Ma, Dongfang Wang, Yingyin Yao, Brian A. Larkins, Qixin Sun, Ramin Yadegari, Xiangfeng Wang, Zhongfu Ni

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

In angiosperms, the endosperm provides nutrients for embryogenesis and seed germination and is the primary tissue where gene imprinting occurs. To identify the imprintome of early developing maize (Zea mays) endosperm, we performed highthroughput transcriptome sequencing of whole kernels at 0, 3, and 5 d after pollination (DAP) and endosperms at 7, 10, and 15 DAP, using B73 byMo17reciprocal crosses.Weobserved gradually increased expression of paternal transcripts in 3- and 5-DAP kernels. In 7-DAP endosperm, the majority of the genes tested reached a 2:1 maternal versus paternal ratio, suggesting that paternal genes are nearly fully activated by 7 DAP. A total of 116, 234, and 63 genes exhibiting parent-specific expression were identified at 7, 10, and 15 DAP, respectively. The largest proportion of paternally expressed genes was at 7 DAP, mainly due to the significantly deviated parental allele expression ratio of these genes at this stage, while nearly 80% of the maternally expressed genes (MEGs) were specific to 10 DAP and were primarily attributed to sharply increased expression levels compared with the other stages. Gene ontology enrichment analysis of the imprinted genes suggested that 10-DAP endosperm-specific MEGs are involved in nutrient uptake and allocation and the auxin signaling pathway, coincident with the onset of starch and storage protein accumulation.

Original languageEnglish (US)
Pages (from-to)3212-3227
Number of pages16
JournalPlant Cell
Volume25
Issue number9
DOIs
StatePublished - Sep 2013

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Pollination
Endosperm
endosperm
Zea mays
pollination
Gene Expression
Food
gene expression
corn
nutrients
Genes
genes
Angiosperms
Gene Ontology
Indoleacetic Acids
genomic imprinting
Germination
Transcriptome
Starch
storage proteins

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Medicine(all)

Cite this

Dynamic expression of imprinted genes associates with maternally controlled nutrient allocation during maize endosperm development. / Xin, Mingming; Yang, Ruolin; Li, Guosheng; Chen, Hao; Laurie, John; Ma, Chuang; Wang, Dongfang; Yao, Yingyin; Larkins, Brian A.; Sun, Qixin; Yadegari, Ramin; Wang, Xiangfeng; Ni, Zhongfu.

In: Plant Cell, Vol. 25, No. 9, 09.2013, p. 3212-3227.

Research output: Contribution to journalArticle

Xin, M, Yang, R, Li, G, Chen, H, Laurie, J, Ma, C, Wang, D, Yao, Y, Larkins, BA, Sun, Q, Yadegari, R, Wang, X & Ni, Z 2013, 'Dynamic expression of imprinted genes associates with maternally controlled nutrient allocation during maize endosperm development', Plant Cell, vol. 25, no. 9, pp. 3212-3227. https://doi.org/10.1105/tpc.113.115592
Xin, Mingming ; Yang, Ruolin ; Li, Guosheng ; Chen, Hao ; Laurie, John ; Ma, Chuang ; Wang, Dongfang ; Yao, Yingyin ; Larkins, Brian A. ; Sun, Qixin ; Yadegari, Ramin ; Wang, Xiangfeng ; Ni, Zhongfu. / Dynamic expression of imprinted genes associates with maternally controlled nutrient allocation during maize endosperm development. In: Plant Cell. 2013 ; Vol. 25, No. 9. pp. 3212-3227.
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