Dynamic organ culture of precision liver slices for in vitro toxicology

P. F. Smith, A. J. Gandolfi, C. L. Krumdieck, C. W. Putnam, C. F. Zukoski, W. M. Davis, K. Brendel

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112 Scopus citations

Abstract

The lack of a reproducible method for the production of thin tissue slices has hindered the use of liver slices as an in vitro tool for hepatotoxicity studies. Fresh human, rat, and rabbit liver was processed using a mechanical slicer. With this instrument, precision (5% of thickness) liver slices in the submillimeter range could be produced at a rapid rate. Slices were prepared from fresh livers in chilled, oxygenated buffer to minimize trauma. Following incubation for up to 20 h in a dynamic organ culture system, histology of incubated slices suggested that 250 m precision-cut slices were optimum in regard to morphology relative to liver slices incubated under conventional organ culture conditions. Addition of bromobenzene to the culture showed time-dependent hepatotoxicity based on two classic parameters of cell degeneration. Histological evidence is presented which suggests the usefulness of this system for hepatotoxicity studies and the production of focal necrosis in vitro.

Original languageEnglish (US)
Pages (from-to)1367-1375
Number of pages9
JournalLife Sciences
Volume36
Issue number14
DOIs
StatePublished - Apr 8 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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    Smith, P. F., Gandolfi, A. J., Krumdieck, C. L., Putnam, C. W., Zukoski, C. F., Davis, W. M., & Brendel, K. (1985). Dynamic organ culture of precision liver slices for in vitro toxicology. Life Sciences, 36(14), 1367-1375. https://doi.org/10.1016/0024-3205(85)90042-6