Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity

Tatsuya Ingi, Andrejs M. Krumins, Peter Chidiac, Greg M. Brothers, Stephen Chung, Bryan E. Snow, Carol A. Barnes, Anthony A. Lanahan, David P. Siderovski, Elliott M. Ross, Alfred G. Gilman, Paul F. Worley

Research output: Contribution to journalArticle

249 Scopus citations

Abstract

Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the a subunit of G(q) and G(i)1. The effect on G(i)1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both G(q)- and G(i)- dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.

Original languageEnglish (US)
Pages (from-to)7178-7188
Number of pages11
JournalJournal of Neuroscience
Volume18
Issue number18
DOIs
StatePublished - Sep 15 1998

Keywords

  • G-protein
  • GTPase- activating proteins
  • Immediate early genes
  • MAP kinase
  • Neuronal plasticity
  • RGS
  • Seizure

ASJC Scopus subject areas

  • Neuroscience(all)

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    Ingi, T., Krumins, A. M., Chidiac, P., Brothers, G. M., Chung, S., Snow, B. E., Barnes, C. A., Lanahan, A. A., Siderovski, D. P., Ross, E. M., Gilman, A. G., & Worley, P. F. (1998). Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity. Journal of Neuroscience, 18(18), 7178-7188. https://doi.org/10.1523/jneurosci.18-18-07178.1998