Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane

Weiwei Xiang, Bettina Reglin, Bianca Nitzsche, Martin Maibier, Wen Wei Rong, Björn Hoffmann, Alfredo Ruggeri, Pedro Guimarães, Timothy W Secomb, Axel R. Pries

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: After arteriolar occlusion, collaterals enlarge and initially elevated WSS normalizes. While most previous studies focused on endpoints of such adaptive changes in larger collaterals, the present investigation aimed to continuously determine the relation between WSS and diameter in microvascular collaterals during adaptive reactions. Methods: In Hamburger-Hamilton stage 40 CAMs, junction points between arteriolar segments were identified and the third upstream segment on one side was occluded. Intravital microscopy recordings were taken for 24 hours post-occlusion. Segment diameter and blood velocity were measured: WSS and capillary density were calculated. Results: After occlusion, vascular diameters exhibited an immediate decrease, then increased with a time constant of 2.5 ± 0.8 hours and reached a plateau of up to 60% above baseline after about 7 hours. Vascular tone showed no significant change. WSS exhibited an immediate increase post-occlusion and linearly returned to baseline after about 12 hours. Local WSS change and diameter change rate showed similar patterns during the initial but not the later phase of post-occlusive adaptation. Conclusions: CAM collaterals undergo fast structural remodeling within 24 hours post-occlusion. This remodeling might be driven by local WSS and by other regulators within the vascular network.

Original languageEnglish (US)
Article numbere12351
JournalMicrocirculation
Volume24
Issue number4
DOIs
StatePublished - May 1 2017

Fingerprint

Chorioallantoic Membrane
Blood Vessels

Keywords

  • collateral diameter adaptation
  • collateral flow
  • micro-occlusion
  • microcirculation
  • wall shear stress

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Xiang, W., Reglin, B., Nitzsche, B., Maibier, M., Rong, W. W., Hoffmann, B., ... Pries, A. R. (2017). Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane. Microcirculation, 24(4), [e12351]. https://doi.org/10.1111/micc.12351

Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane. / Xiang, Weiwei; Reglin, Bettina; Nitzsche, Bianca; Maibier, Martin; Rong, Wen Wei; Hoffmann, Björn; Ruggeri, Alfredo; Guimarães, Pedro; Secomb, Timothy W; Pries, Axel R.

In: Microcirculation, Vol. 24, No. 4, e12351, 01.05.2017.

Research output: Contribution to journalArticle

Xiang, W, Reglin, B, Nitzsche, B, Maibier, M, Rong, WW, Hoffmann, B, Ruggeri, A, Guimarães, P, Secomb, TW & Pries, AR 2017, 'Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane', Microcirculation, vol. 24, no. 4, e12351. https://doi.org/10.1111/micc.12351
Xiang, Weiwei ; Reglin, Bettina ; Nitzsche, Bianca ; Maibier, Martin ; Rong, Wen Wei ; Hoffmann, Björn ; Ruggeri, Alfredo ; Guimarães, Pedro ; Secomb, Timothy W ; Pries, Axel R. / Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane. In: Microcirculation. 2017 ; Vol. 24, No. 4.
@article{593c47bebba44ea1a601eda768ee12e8,
title = "Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane",
abstract = "Objective: After arteriolar occlusion, collaterals enlarge and initially elevated WSS normalizes. While most previous studies focused on endpoints of such adaptive changes in larger collaterals, the present investigation aimed to continuously determine the relation between WSS and diameter in microvascular collaterals during adaptive reactions. Methods: In Hamburger-Hamilton stage 40 CAMs, junction points between arteriolar segments were identified and the third upstream segment on one side was occluded. Intravital microscopy recordings were taken for 24 hours post-occlusion. Segment diameter and blood velocity were measured: WSS and capillary density were calculated. Results: After occlusion, vascular diameters exhibited an immediate decrease, then increased with a time constant of 2.5 ± 0.8 hours and reached a plateau of up to 60{\%} above baseline after about 7 hours. Vascular tone showed no significant change. WSS exhibited an immediate increase post-occlusion and linearly returned to baseline after about 12 hours. Local WSS change and diameter change rate showed similar patterns during the initial but not the later phase of post-occlusive adaptation. Conclusions: CAM collaterals undergo fast structural remodeling within 24 hours post-occlusion. This remodeling might be driven by local WSS and by other regulators within the vascular network.",
keywords = "collateral diameter adaptation, collateral flow, micro-occlusion, microcirculation, wall shear stress",
author = "Weiwei Xiang and Bettina Reglin and Bianca Nitzsche and Martin Maibier and Rong, {Wen Wei} and Bj{\"o}rn Hoffmann and Alfredo Ruggeri and Pedro Guimar{\~a}es and Secomb, {Timothy W} and Pries, {Axel R.}",
year = "2017",
month = "5",
day = "1",
doi = "10.1111/micc.12351",
language = "English (US)",
volume = "24",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane

AU - Xiang, Weiwei

AU - Reglin, Bettina

AU - Nitzsche, Bianca

AU - Maibier, Martin

AU - Rong, Wen Wei

AU - Hoffmann, Björn

AU - Ruggeri, Alfredo

AU - Guimarães, Pedro

AU - Secomb, Timothy W

AU - Pries, Axel R.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Objective: After arteriolar occlusion, collaterals enlarge and initially elevated WSS normalizes. While most previous studies focused on endpoints of such adaptive changes in larger collaterals, the present investigation aimed to continuously determine the relation between WSS and diameter in microvascular collaterals during adaptive reactions. Methods: In Hamburger-Hamilton stage 40 CAMs, junction points between arteriolar segments were identified and the third upstream segment on one side was occluded. Intravital microscopy recordings were taken for 24 hours post-occlusion. Segment diameter and blood velocity were measured: WSS and capillary density were calculated. Results: After occlusion, vascular diameters exhibited an immediate decrease, then increased with a time constant of 2.5 ± 0.8 hours and reached a plateau of up to 60% above baseline after about 7 hours. Vascular tone showed no significant change. WSS exhibited an immediate increase post-occlusion and linearly returned to baseline after about 12 hours. Local WSS change and diameter change rate showed similar patterns during the initial but not the later phase of post-occlusive adaptation. Conclusions: CAM collaterals undergo fast structural remodeling within 24 hours post-occlusion. This remodeling might be driven by local WSS and by other regulators within the vascular network.

AB - Objective: After arteriolar occlusion, collaterals enlarge and initially elevated WSS normalizes. While most previous studies focused on endpoints of such adaptive changes in larger collaterals, the present investigation aimed to continuously determine the relation between WSS and diameter in microvascular collaterals during adaptive reactions. Methods: In Hamburger-Hamilton stage 40 CAMs, junction points between arteriolar segments were identified and the third upstream segment on one side was occluded. Intravital microscopy recordings were taken for 24 hours post-occlusion. Segment diameter and blood velocity were measured: WSS and capillary density were calculated. Results: After occlusion, vascular diameters exhibited an immediate decrease, then increased with a time constant of 2.5 ± 0.8 hours and reached a plateau of up to 60% above baseline after about 7 hours. Vascular tone showed no significant change. WSS exhibited an immediate increase post-occlusion and linearly returned to baseline after about 12 hours. Local WSS change and diameter change rate showed similar patterns during the initial but not the later phase of post-occlusive adaptation. Conclusions: CAM collaterals undergo fast structural remodeling within 24 hours post-occlusion. This remodeling might be driven by local WSS and by other regulators within the vascular network.

KW - collateral diameter adaptation

KW - collateral flow

KW - micro-occlusion

KW - microcirculation

KW - wall shear stress

UR - http://www.scopus.com/inward/record.url?scp=85019221975&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019221975&partnerID=8YFLogxK

U2 - 10.1111/micc.12351

DO - 10.1111/micc.12351

M3 - Article

C2 - 28075525

AN - SCOPUS:85019221975

VL - 24

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 4

M1 - e12351

ER -