Dynamics of genome evolution in facultative symbionts of aphids

Patrick H. Degnan, Teresa E. Leonardo, Bodil N. Cass, Bonnie L Hurwitz, David Stern, Richard A. Gibbs, Stephen Richards, Nancy A. Moran

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Aphids are sap-feeding insects that host a range of bacterial endosymbionts including the obligate, nutritional mutualist Buchnera plus several bacteria that are not required for host survival. Among the latter, 'Candidatus Regiella insecticola' and 'Candidatus Hamiltonella defensa' are found in pea aphids and other hosts and have been shown to protect aphids from natural enemies. We have sequenced almost the entire genome of R. insecticola (2.07 Mbp) and compared it with the recently published genome of H. defensa (2.11 Mbp). Despite being sister species the two genomes are highly rearranged and the genomes only have ∼55% of genes in common. The functions encoded by the shared genes imply that the bacteria have similar metabolic capabilities, including only two essential amino acid biosynthetic pathways and active uptake mechanisms for the remaining eight, and similar capacities for host cell toxicity and invasion (type 3 secretion systems and RTX toxins). These observations, combined with high sequence divergence of orthologues, strongly suggest an ancient divergence after establishment of a symbiotic lifestyle. The divergence in gene sets and in genome architecture implies a history of rampant recombination and gene inactivation and the ongoing integration of mobile DNA (insertion sequence elements, prophage and plasmids).

Original languageEnglish (US)
Pages (from-to)2060-2069
Number of pages10
JournalEnvironmental Microbiology
Volume12
Issue number8
DOIs
StatePublished - Aug 2010

Fingerprint

Aphids
symbiont
aphid
symbionts
Aphidoidea
genome
Genome
DNA Transposable Elements
gene
divergence
Buchnera
genes
Genes
Bacteria
Prophages
type III secretion system
bacterium
cell invasion
Acyrthosiphon pisum
Essential Amino Acids

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Degnan, P. H., Leonardo, T. E., Cass, B. N., Hurwitz, B. L., Stern, D., Gibbs, R. A., ... Moran, N. A. (2010). Dynamics of genome evolution in facultative symbionts of aphids. Environmental Microbiology, 12(8), 2060-2069. https://doi.org/10.1111/j.1462-2920.2009.02085.x

Dynamics of genome evolution in facultative symbionts of aphids. / Degnan, Patrick H.; Leonardo, Teresa E.; Cass, Bodil N.; Hurwitz, Bonnie L; Stern, David; Gibbs, Richard A.; Richards, Stephen; Moran, Nancy A.

In: Environmental Microbiology, Vol. 12, No. 8, 08.2010, p. 2060-2069.

Research output: Contribution to journalArticle

Degnan, PH, Leonardo, TE, Cass, BN, Hurwitz, BL, Stern, D, Gibbs, RA, Richards, S & Moran, NA 2010, 'Dynamics of genome evolution in facultative symbionts of aphids', Environmental Microbiology, vol. 12, no. 8, pp. 2060-2069. https://doi.org/10.1111/j.1462-2920.2009.02085.x
Degnan, Patrick H. ; Leonardo, Teresa E. ; Cass, Bodil N. ; Hurwitz, Bonnie L ; Stern, David ; Gibbs, Richard A. ; Richards, Stephen ; Moran, Nancy A. / Dynamics of genome evolution in facultative symbionts of aphids. In: Environmental Microbiology. 2010 ; Vol. 12, No. 8. pp. 2060-2069.
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