Dynamics of growth zone patterning in the milkweed bug Oncopeltus fasciatus

Tzach Auman, Barbara M.I. Vreede, Aryeh Weiss, Susan D. Hester, Terri A. Williams, Lisa M. Nagy, Ariel D. Chipman

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We describe the dynamic process of abdominal segment generation in the milkweed bug Oncopeltus fasciatus. We present detailed morphological measurements of the growing germband throughout segmentation. Our data are complemented by cell division profiles and expression patterns of key genes, including invected and even-skipped as markers for different stages of segment formation. We describe morphological and mechanistic changes in the growth zone and in nascent segments during the generation of individual segments and throughout segmentation, and examine the relative contribution of newly formed versus existing tissue to segment formation. Although abdominal segment addition is primarily generated through the rearrangement of a pool of undifferentiated cells, there is nonetheless proliferation in the posterior. By correlating proliferation with gene expression in the growth zone, we propose a model for growth zone dynamics during segmentation in which the growth zone is functionally subdivided into two distinct regions: a posterior region devoted to a slow rate of growth among undifferentiated cells, and an anterior region in which segmental differentiation is initiated and proliferation inhibited.

Original languageEnglish (US)
Pages (from-to)1896-1905
Number of pages10
JournalDevelopment (Cambridge)
Volume144
Issue number10
DOIs
StatePublished - May 15 2017

Keywords

  • Arthropod
  • Cell division
  • Growth zone
  • Segmentation
  • Terminal addition

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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