Dynamics of lipid bilayers from comparative analysis of 2H and 13C nuclear magnetic resonance relaxation data as a function of frequency and temperature

Alexander A. Nevzorov, Michael F. Brown

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Analysis of the nuclear spin relaxation rates of lipid membranes provides a powerful means of studying the dynamics of these important biological representatives of soft matter. Here, temperature- and frequency-dependent 2H and 13C nuclear magnetic resonance (NMR) relaxation rates for vesicles and multilamellar dispersions of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in the liquid-crystalline state have been fitted simultaneously to various dynamic models for different positions of the acyl chains. The data include 2H R1z rates (Zeeman order of electric quadrupolar interaction) acquired at 12 external magnetic field strengths from 0.382 to 14.6 T, corresponding to a frequency range from ωD/2π=2.50-95.3 MHz; and 2H R1Q rates (quadrupolar order of electric quadrupolar interaction) at 15.3, 46.1, and 76.8 MHz. Moreover, 13C R1z data (Zeeman order of magnetic dipolar interaction) for DMPC are included at six magnetic field strengths, ranging from 1.40 to 17.6 T, thereby enabling extension of the frequency range to effectively (ωCH)/2π=938.7 MHz. Use of the generalized approach allows formulation of noncollective segmental and molecular diffusion models, as well as collective director fluctuation models, which were tested by fitting the 2H R1Z data at different frequencies and temperatures (30 °C and 50 °C). The corresponding 13C relaxation rates were predicted theoretically and compared to experiment, thus allowing one to unify the 13C and 2H NMR data for bilayer lipids in the fluid state. A further new aspect is that the spectral densities of motion have been explicitly calculated from the 2H R1Z and R1Q data at 40 °C. We conclude that the relaxation in fluid membrane bilayers is governed predominantly by relatively slow motions, which modulate the residual coupling remaining from faster local motions (order fluctuations). Only the molecular diffusion model, including an additional slow motional process, and the membrane deformation model describing three-dimensional collective fluctuations fit the 2H NMR data and predict the 13C NMR data in the MHz range. Orientational correlation functions have been calculated, which emphasizes the importance of NMR relaxation as a unique tool for investigating the dynamics of lipid bilayers and biological membranes.

Original languageEnglish (US)
Pages (from-to)10288-10310
Number of pages23
JournalJournal of Chemical Physics
Volume107
Issue number23
DOIs
StatePublished - Dec 15 1997

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Fingerprint Dive into the research topics of 'Dynamics of lipid bilayers from comparative analysis of <sup>2</sup>H and <sup>13</sup>C nuclear magnetic resonance relaxation data as a function of frequency and temperature'. Together they form a unique fingerprint.

  • Cite this