Dynamics of Neisseria gonorrhoeae attachment: Microcolony development, cortical plaque formation, and cytoprotection

Dustin L. Higashi, Shaun W. Lee, Aurelie Snyder, Nathan J. Weyand, Antony Bakke, Magdalene So

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Neisseria gonorrhoeae is the bacterium that causes gonorrhea, a major sexually transmitted disease and a significant cofactor for human immunodeficiency virus transmission. The retactile N. gonorrhoeae type IV pilus (Tfp) mediates twitching motility and attachment. Using live-cell microscopy, we reveal for the first time the dynamics of twitching motility by N. gonorrhoeae in its natural environment, human epithelial cells. Bacteria aggregate into microcolonies on the cell surface and induce a massive remodeling of the microvillus architecture. Surprisingly, the microcolonies are motile, and they fuse to form progressively larger structures that undergo rapid reorganization, suggesting that bacteria communicate with each other during infection. As reported, actin plaques form beneath microcolonies. Here, we show that cortical plaques comigrate with motile microcolonies. These activities are dependent on pilT, the Tfp retraction locus. Cultures infected with a pilT mutant have significantly higher numbers of apoptotic cells than cultures infected with the wild-type strain. Inducing pilT expression with isopropyl-β-D- thiogalactopyranoside partially rescues cells from infection-induced apoptosis, demonstrating that Tfp retraction is intrinsically cytoprotective for the host. Tfp-mediated attachment is therefore a continuum of microcolony motility and force stimulation of host cell signaling, leading to a cytoprotective effect.

Original languageEnglish (US)
Pages (from-to)4743-4753
Number of pages11
JournalInfection and Immunity
Volume75
Issue number10
DOIs
StatePublished - Oct 2007

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Dynamics of Neisseria gonorrhoeae attachment: Microcolony development, cortical plaque formation, and cytoprotection'. Together they form a unique fingerprint.

  • Cite this