E- and P-selectin expression depends on the resuscitation fluid used in hemorrhaged rats

H. B. Alam, L. Sun, P. Ruff, B. Austin, D. Burris, Peter M Rhee

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Background. E- and P-selectins are adhesion molecules that effect neutrophil-mediated reperfusion injury. Our hypothesis was that the expression of E- and P-selectins is dependent on the type of fluid used for resuscitation and that lactated Ringer's (LR) solution would result in an early upregulation of these molecules. Methods. Male Sprague-Dawley rats (n = 36) were subjected to a 27 ml/kg hemorrhage over 5 min followed by a 1-h shock period and 1-h of resuscitation. The animals were randomized into the following resuscitation groups: (1) sham; (2) hemorrhage, no resuscitation; (3) whole blood (27 ml/kg); (4) 3:1 lactated Ringer's (81 ml/ kg); (5) sham hemorrhage, infusion of lactated Ringer's (81 ml/kg); (6) 7.5% hypertonic saline (9.7 ml/kg). Immediately after resuscitation, the spleen and lung were harvested for measurement of E- and P-selectin mRNA expression with reverse transcriptase- polymerase chain reaction (RT-PCR), and protein expression with immunostaining. Results. LR resuscitation and LR infusion without prior hemorrhage significantly increased the E- and P-selectin mRNA in the lung and spleen. Immunostaining demonstrated that the adhesion molecule expression was mainly located in perivascular/peribronchial areas in the lung, and the marginal and trabecular areas in the spleen. Pulmonary edema and inflammatory cell infiltration were observed only in the animals that were hemorrhaged and resuscitated with LR. No resuscitation and resuscitation with whole blood caused no significant increase in selectin expression. Conclusion. LR resuscitation and LR infusion without hemorrhage are associated with early increased expression of E- and P-selectin molecules in the lung and spleen. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalJournal of Surgical Research
Volume94
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

P-Selectin
E-Selectin
Resuscitation
Hemorrhage
Spleen
Lung
Selectins
Messenger RNA
Ringer's lactate
Pulmonary Edema
Reperfusion Injury
Reverse Transcriptase Polymerase Chain Reaction
Sprague Dawley Rats
Shock
Neutrophils
Up-Regulation

Keywords

  • Adhesion
  • E-selectin
  • Hemorrhagic shock
  • Hypertonic saline
  • Lactated Ringer's
  • Lungs
  • P-selectin
  • Rats
  • Resuscitation
  • Spleen

ASJC Scopus subject areas

  • Surgery

Cite this

E- and P-selectin expression depends on the resuscitation fluid used in hemorrhaged rats. / Alam, H. B.; Sun, L.; Ruff, P.; Austin, B.; Burris, D.; Rhee, Peter M.

In: Journal of Surgical Research, Vol. 94, No. 2, 2000, p. 145-152.

Research output: Contribution to journalArticle

Alam, H. B. ; Sun, L. ; Ruff, P. ; Austin, B. ; Burris, D. ; Rhee, Peter M. / E- and P-selectin expression depends on the resuscitation fluid used in hemorrhaged rats. In: Journal of Surgical Research. 2000 ; Vol. 94, No. 2. pp. 145-152.
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abstract = "Background. E- and P-selectins are adhesion molecules that effect neutrophil-mediated reperfusion injury. Our hypothesis was that the expression of E- and P-selectins is dependent on the type of fluid used for resuscitation and that lactated Ringer's (LR) solution would result in an early upregulation of these molecules. Methods. Male Sprague-Dawley rats (n = 36) were subjected to a 27 ml/kg hemorrhage over 5 min followed by a 1-h shock period and 1-h of resuscitation. The animals were randomized into the following resuscitation groups: (1) sham; (2) hemorrhage, no resuscitation; (3) whole blood (27 ml/kg); (4) 3:1 lactated Ringer's (81 ml/ kg); (5) sham hemorrhage, infusion of lactated Ringer's (81 ml/kg); (6) 7.5{\%} hypertonic saline (9.7 ml/kg). Immediately after resuscitation, the spleen and lung were harvested for measurement of E- and P-selectin mRNA expression with reverse transcriptase- polymerase chain reaction (RT-PCR), and protein expression with immunostaining. Results. LR resuscitation and LR infusion without prior hemorrhage significantly increased the E- and P-selectin mRNA in the lung and spleen. Immunostaining demonstrated that the adhesion molecule expression was mainly located in perivascular/peribronchial areas in the lung, and the marginal and trabecular areas in the spleen. Pulmonary edema and inflammatory cell infiltration were observed only in the animals that were hemorrhaged and resuscitated with LR. No resuscitation and resuscitation with whole blood caused no significant increase in selectin expression. Conclusion. LR resuscitation and LR infusion without hemorrhage are associated with early increased expression of E- and P-selectin molecules in the lung and spleen. (C) 2000 Academic Press.",
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T1 - E- and P-selectin expression depends on the resuscitation fluid used in hemorrhaged rats

AU - Alam, H. B.

AU - Sun, L.

AU - Ruff, P.

AU - Austin, B.

AU - Burris, D.

AU - Rhee, Peter M

PY - 2000

Y1 - 2000

N2 - Background. E- and P-selectins are adhesion molecules that effect neutrophil-mediated reperfusion injury. Our hypothesis was that the expression of E- and P-selectins is dependent on the type of fluid used for resuscitation and that lactated Ringer's (LR) solution would result in an early upregulation of these molecules. Methods. Male Sprague-Dawley rats (n = 36) were subjected to a 27 ml/kg hemorrhage over 5 min followed by a 1-h shock period and 1-h of resuscitation. The animals were randomized into the following resuscitation groups: (1) sham; (2) hemorrhage, no resuscitation; (3) whole blood (27 ml/kg); (4) 3:1 lactated Ringer's (81 ml/ kg); (5) sham hemorrhage, infusion of lactated Ringer's (81 ml/kg); (6) 7.5% hypertonic saline (9.7 ml/kg). Immediately after resuscitation, the spleen and lung were harvested for measurement of E- and P-selectin mRNA expression with reverse transcriptase- polymerase chain reaction (RT-PCR), and protein expression with immunostaining. Results. LR resuscitation and LR infusion without prior hemorrhage significantly increased the E- and P-selectin mRNA in the lung and spleen. Immunostaining demonstrated that the adhesion molecule expression was mainly located in perivascular/peribronchial areas in the lung, and the marginal and trabecular areas in the spleen. Pulmonary edema and inflammatory cell infiltration were observed only in the animals that were hemorrhaged and resuscitated with LR. No resuscitation and resuscitation with whole blood caused no significant increase in selectin expression. Conclusion. LR resuscitation and LR infusion without hemorrhage are associated with early increased expression of E- and P-selectin molecules in the lung and spleen. (C) 2000 Academic Press.

AB - Background. E- and P-selectins are adhesion molecules that effect neutrophil-mediated reperfusion injury. Our hypothesis was that the expression of E- and P-selectins is dependent on the type of fluid used for resuscitation and that lactated Ringer's (LR) solution would result in an early upregulation of these molecules. Methods. Male Sprague-Dawley rats (n = 36) were subjected to a 27 ml/kg hemorrhage over 5 min followed by a 1-h shock period and 1-h of resuscitation. The animals were randomized into the following resuscitation groups: (1) sham; (2) hemorrhage, no resuscitation; (3) whole blood (27 ml/kg); (4) 3:1 lactated Ringer's (81 ml/ kg); (5) sham hemorrhage, infusion of lactated Ringer's (81 ml/kg); (6) 7.5% hypertonic saline (9.7 ml/kg). Immediately after resuscitation, the spleen and lung were harvested for measurement of E- and P-selectin mRNA expression with reverse transcriptase- polymerase chain reaction (RT-PCR), and protein expression with immunostaining. Results. LR resuscitation and LR infusion without prior hemorrhage significantly increased the E- and P-selectin mRNA in the lung and spleen. Immunostaining demonstrated that the adhesion molecule expression was mainly located in perivascular/peribronchial areas in the lung, and the marginal and trabecular areas in the spleen. Pulmonary edema and inflammatory cell infiltration were observed only in the animals that were hemorrhaged and resuscitated with LR. No resuscitation and resuscitation with whole blood caused no significant increase in selectin expression. Conclusion. LR resuscitation and LR infusion without hemorrhage are associated with early increased expression of E- and P-selectin molecules in the lung and spleen. (C) 2000 Academic Press.

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KW - Hemorrhagic shock

KW - Hypertonic saline

KW - Lactated Ringer's

KW - Lungs

KW - P-selectin

KW - Rats

KW - Resuscitation

KW - Spleen

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