Early alterations of lung injury following acute smoke exposure and 21- aminosteroid treatment

Shengjun Wang, Robert Clark Lantz, Raymond F. Robledo, Veronica Breceda, Allison M. Hays, Mark L. Witten

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In a simulated fire-related smoke exposure protocol, New Zealand white rabbits were utilized to investigate the potential effects of the 21- aminosteroid (lazaroid) analog U75412E on the early events of acute lung injury. Inhalation of a total of 1.6 mg/kg U75412E aerosolized at a rate of 1.53 mg/min at 0.5 hr after smoke exposure significantly attenuated the extent of lung injury at 1 hr, as evidenced by decreased bronchoalveolar lavage (BAL) concentration of total protein, 6-keto-prostaglandin F1-α, and blood gas defect. Histopathologic examination demonstrated that the lazaroid significantly attenuated smoke-induced lung injury as evidenced by a decrease in wet lung/body weight ratio, necrosis, and sloughing of airway epithelial cells. Electron microscopy showed that the lazaroid decreased smoke-induced interstitial edema and the vacuolization of alveolar type II epithelium (21.6 ± 9.7 vs 8.5 ± 3.6 vacuoled blebs/cell, smoke only vs smoke + lazaroid). However, U75412E did not attenuate smoke-induced changes in BAL concentration of tumor necrosis factor-α, total cell count, and granulocyte percentage. These observations suggest that U75412E may exert its action through cooperative mechanisms, such as the modulation of arachidonic acid metabolism, in addition to its characterized antioxidative effects.

Original languageEnglish (US)
Pages (from-to)334-341
Number of pages8
JournalToxicologic Pathology
Volume27
Issue number3
StatePublished - 1999

Fingerprint

Acute Lung Injury
Smoke
Lung Injury
Bronchoalveolar Lavage
Therapeutics
Pulmonary Edema
Blister
Granulocytes
Arachidonic Acid
Metabolism
Inhalation
Electron microscopy
Edema
Electron Microscopy
Fires
Blood
Necrosis
Epithelium
Tumor Necrosis Factor-alpha
Cell Count

Keywords

  • Gas exchange
  • Lazaroids
  • Prostaglandin
  • Respiratory epithelium
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Wang, S., Lantz, R. C., Robledo, R. F., Breceda, V., Hays, A. M., & Witten, M. L. (1999). Early alterations of lung injury following acute smoke exposure and 21- aminosteroid treatment. Toxicologic Pathology, 27(3), 334-341.

Early alterations of lung injury following acute smoke exposure and 21- aminosteroid treatment. / Wang, Shengjun; Lantz, Robert Clark; Robledo, Raymond F.; Breceda, Veronica; Hays, Allison M.; Witten, Mark L.

In: Toxicologic Pathology, Vol. 27, No. 3, 1999, p. 334-341.

Research output: Contribution to journalArticle

Wang, S, Lantz, RC, Robledo, RF, Breceda, V, Hays, AM & Witten, ML 1999, 'Early alterations of lung injury following acute smoke exposure and 21- aminosteroid treatment', Toxicologic Pathology, vol. 27, no. 3, pp. 334-341.
Wang, Shengjun ; Lantz, Robert Clark ; Robledo, Raymond F. ; Breceda, Veronica ; Hays, Allison M. ; Witten, Mark L. / Early alterations of lung injury following acute smoke exposure and 21- aminosteroid treatment. In: Toxicologic Pathology. 1999 ; Vol. 27, No. 3. pp. 334-341.
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