Early but not late burn wound excision partially restores viral-specific T lymphocyte cytotoxicity

C. Scott Hultman, Hiromasa Yamamoto, Suzan DeSerres, Jeffrey A Frelinger, Anthony A. Meyer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: Early burn wound excision restores immunocompetence and improves patient survival, but the exact mechanisms have not yet been defined. Burn injury impairs cytotoxic T lymphocyte (CTL) activity as a function of burn size, increasing the risk of infection. The purpose of this study was to determine if early wound excision improved viral-specific CTL function. Methods: Anesthetized C57BL/6 mice (n = 20) received 0%, 20%, or 40% total body surface area full-thickness contact burns and were inoculated 3 days later with intraperitoneal lymphocytic choriomeningitis virus. Eight days after infection, or 11 days after burn, CTL effectors (E) were harvested and tested against infected, radiolabeled L-Db targets (T) in a 51Cr- release assay, at varied E:T ratios. Dilution curves of CTL activity were compared by analysis of variance. In the second experiment, mice (n = 18) underwent a 30% burn that was totally excised and grafted on postburn days (PBDs) 0, 3, and 7. Control groups included sham burn and no excision of a 30% burn. In the third experiment, mice (n = 22) received a 30% burn that was partially, completely, or not excised on PBD 3. Control groups included sham burn with and without excision. All groups were infected with intraperitoneal lymphocytic choriomeningitis virus on PBD 3. Viral-specific CTL activity was determined on PBD 11. Results: Both 20% and 40% burn injury impaired viral- specific CTL function. Wound excision on PBDs 0 and 3, but not on PBD 7, partially restored CTL function. Total excision of the 30% burn improved CTL activity to a greater extent than did partial excision. Conclusion: Burn injury inhibits viral-specific CTL activity. Early, complete wound excision augments CTL function. Improved CTL activity after burn may reduce the risk of infection, providing an immunologic rationale for expeditious wound excision.

Original languageEnglish (US)
Pages (from-to)441-447
Number of pages7
JournalJournal of Trauma
Volume43
Issue number3
DOIs
StatePublished - Sep 1997
Externally publishedYes

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Cytotoxic T-Lymphocytes
T-Lymphocytes
Wounds and Injuries
Lymphocytic choriomeningitis virus
Infection
Immunocompetence
Control Groups
Body Surface Area
Burns
Inbred C57BL Mouse
Analysis of Variance

ASJC Scopus subject areas

  • Surgery

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Early but not late burn wound excision partially restores viral-specific T lymphocyte cytotoxicity. / Hultman, C. Scott; Yamamoto, Hiromasa; DeSerres, Suzan; Frelinger, Jeffrey A; Meyer, Anthony A.

In: Journal of Trauma, Vol. 43, No. 3, 09.1997, p. 441-447.

Research output: Contribution to journalArticle

Hultman, C. Scott ; Yamamoto, Hiromasa ; DeSerres, Suzan ; Frelinger, Jeffrey A ; Meyer, Anthony A. / Early but not late burn wound excision partially restores viral-specific T lymphocyte cytotoxicity. In: Journal of Trauma. 1997 ; Vol. 43, No. 3. pp. 441-447.
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title = "Early but not late burn wound excision partially restores viral-specific T lymphocyte cytotoxicity",
abstract = "Objective: Early burn wound excision restores immunocompetence and improves patient survival, but the exact mechanisms have not yet been defined. Burn injury impairs cytotoxic T lymphocyte (CTL) activity as a function of burn size, increasing the risk of infection. The purpose of this study was to determine if early wound excision improved viral-specific CTL function. Methods: Anesthetized C57BL/6 mice (n = 20) received 0{\%}, 20{\%}, or 40{\%} total body surface area full-thickness contact burns and were inoculated 3 days later with intraperitoneal lymphocytic choriomeningitis virus. Eight days after infection, or 11 days after burn, CTL effectors (E) were harvested and tested against infected, radiolabeled L-Db targets (T) in a 51Cr- release assay, at varied E:T ratios. Dilution curves of CTL activity were compared by analysis of variance. In the second experiment, mice (n = 18) underwent a 30{\%} burn that was totally excised and grafted on postburn days (PBDs) 0, 3, and 7. Control groups included sham burn and no excision of a 30{\%} burn. In the third experiment, mice (n = 22) received a 30{\%} burn that was partially, completely, or not excised on PBD 3. Control groups included sham burn with and without excision. All groups were infected with intraperitoneal lymphocytic choriomeningitis virus on PBD 3. Viral-specific CTL activity was determined on PBD 11. Results: Both 20{\%} and 40{\%} burn injury impaired viral- specific CTL function. Wound excision on PBDs 0 and 3, but not on PBD 7, partially restored CTL function. Total excision of the 30{\%} burn improved CTL activity to a greater extent than did partial excision. Conclusion: Burn injury inhibits viral-specific CTL activity. Early, complete wound excision augments CTL function. Improved CTL activity after burn may reduce the risk of infection, providing an immunologic rationale for expeditious wound excision.",
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T1 - Early but not late burn wound excision partially restores viral-specific T lymphocyte cytotoxicity

AU - Hultman, C. Scott

AU - Yamamoto, Hiromasa

AU - DeSerres, Suzan

AU - Frelinger, Jeffrey A

AU - Meyer, Anthony A.

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N2 - Objective: Early burn wound excision restores immunocompetence and improves patient survival, but the exact mechanisms have not yet been defined. Burn injury impairs cytotoxic T lymphocyte (CTL) activity as a function of burn size, increasing the risk of infection. The purpose of this study was to determine if early wound excision improved viral-specific CTL function. Methods: Anesthetized C57BL/6 mice (n = 20) received 0%, 20%, or 40% total body surface area full-thickness contact burns and were inoculated 3 days later with intraperitoneal lymphocytic choriomeningitis virus. Eight days after infection, or 11 days after burn, CTL effectors (E) were harvested and tested against infected, radiolabeled L-Db targets (T) in a 51Cr- release assay, at varied E:T ratios. Dilution curves of CTL activity were compared by analysis of variance. In the second experiment, mice (n = 18) underwent a 30% burn that was totally excised and grafted on postburn days (PBDs) 0, 3, and 7. Control groups included sham burn and no excision of a 30% burn. In the third experiment, mice (n = 22) received a 30% burn that was partially, completely, or not excised on PBD 3. Control groups included sham burn with and without excision. All groups were infected with intraperitoneal lymphocytic choriomeningitis virus on PBD 3. Viral-specific CTL activity was determined on PBD 11. Results: Both 20% and 40% burn injury impaired viral- specific CTL function. Wound excision on PBDs 0 and 3, but not on PBD 7, partially restored CTL function. Total excision of the 30% burn improved CTL activity to a greater extent than did partial excision. Conclusion: Burn injury inhibits viral-specific CTL activity. Early, complete wound excision augments CTL function. Improved CTL activity after burn may reduce the risk of infection, providing an immunologic rationale for expeditious wound excision.

AB - Objective: Early burn wound excision restores immunocompetence and improves patient survival, but the exact mechanisms have not yet been defined. Burn injury impairs cytotoxic T lymphocyte (CTL) activity as a function of burn size, increasing the risk of infection. The purpose of this study was to determine if early wound excision improved viral-specific CTL function. Methods: Anesthetized C57BL/6 mice (n = 20) received 0%, 20%, or 40% total body surface area full-thickness contact burns and were inoculated 3 days later with intraperitoneal lymphocytic choriomeningitis virus. Eight days after infection, or 11 days after burn, CTL effectors (E) were harvested and tested against infected, radiolabeled L-Db targets (T) in a 51Cr- release assay, at varied E:T ratios. Dilution curves of CTL activity were compared by analysis of variance. In the second experiment, mice (n = 18) underwent a 30% burn that was totally excised and grafted on postburn days (PBDs) 0, 3, and 7. Control groups included sham burn and no excision of a 30% burn. In the third experiment, mice (n = 22) received a 30% burn that was partially, completely, or not excised on PBD 3. Control groups included sham burn with and without excision. All groups were infected with intraperitoneal lymphocytic choriomeningitis virus on PBD 3. Viral-specific CTL activity was determined on PBD 11. Results: Both 20% and 40% burn injury impaired viral- specific CTL function. Wound excision on PBDs 0 and 3, but not on PBD 7, partially restored CTL function. Total excision of the 30% burn improved CTL activity to a greater extent than did partial excision. Conclusion: Burn injury inhibits viral-specific CTL activity. Early, complete wound excision augments CTL function. Improved CTL activity after burn may reduce the risk of infection, providing an immunologic rationale for expeditious wound excision.

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