Early, complete burn wound excision partially restores cytotoxic T lymphocyte function

C. Scott Hultman, Bruce A. Cairns, Suzan deSerres, Jeffrey A Frelinger, Anthony A. Meyer

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background. Cytotoxic lymphocytes (CTLs) are an important component of immune function, involved in antigen recognition and resistance to viral infection. Burn injury suppresses cell-mediated immunity, induces allograft tolerance, and increases the risk of viral infection, but the mechanisms are not well understood. This study analyzes the effect of burn size and burn wound excision on CTL activity. Methods. Anesthetized CBA mice (n=12) received a 0%, 20%, or 40% body surface area contact burn. Additional mice (n=16) received a 40% burn that was totally, partially, or not excised 72 hours after burn. Excised areas were covered with normal, syngeneic skin. Two weeks later harvested splenocytes were cocultured with allogeneic stimulators. CTL activity was determined by a 51Cr release assay, in which CTL effectors were tested on allogeneic, radiolabeled targets. Dilution curves of CTL activity were compared by ANOVA. Results. Both 20% and 40% burns significantly inhibited CTL activity (p<0.05). Total but not partial excision of a 40% burn restored CTL activity (p<0.01). Both total and partial wound excision also improved survival (p<0.05). Conclusions. Burn injury inhibits CTL activity in a size-dependent manner, and total wound excision significantly improves both CTL function and survival after injury. This study suggests a mechanism for the immunosuppressive effects of burn injury and provides an immunologic rationale for early, complete burn wound excision.

Original languageEnglish (US)
Pages (from-to)421-430
Number of pages10
JournalSurgery
Volume118
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Cytotoxic T-Lymphocytes
Lymphocytes
Wounds and Injuries
Virus Diseases
Transplantation Tolerance
Inbred CBA Mouse
Body Surface Area
Immunosuppressive Agents
Burns
Cellular Immunity
Analysis of Variance
Antigens
Skin

ASJC Scopus subject areas

  • Surgery

Cite this

Early, complete burn wound excision partially restores cytotoxic T lymphocyte function. / Hultman, C. Scott; Cairns, Bruce A.; deSerres, Suzan; Frelinger, Jeffrey A; Meyer, Anthony A.

In: Surgery, Vol. 118, No. 2, 1995, p. 421-430.

Research output: Contribution to journalArticle

Hultman, C. Scott ; Cairns, Bruce A. ; deSerres, Suzan ; Frelinger, Jeffrey A ; Meyer, Anthony A. / Early, complete burn wound excision partially restores cytotoxic T lymphocyte function. In: Surgery. 1995 ; Vol. 118, No. 2. pp. 421-430.
@article{bf30d077ace94c89aa40523117f6a3a8,
title = "Early, complete burn wound excision partially restores cytotoxic T lymphocyte function",
abstract = "Background. Cytotoxic lymphocytes (CTLs) are an important component of immune function, involved in antigen recognition and resistance to viral infection. Burn injury suppresses cell-mediated immunity, induces allograft tolerance, and increases the risk of viral infection, but the mechanisms are not well understood. This study analyzes the effect of burn size and burn wound excision on CTL activity. Methods. Anesthetized CBA mice (n=12) received a 0{\%}, 20{\%}, or 40{\%} body surface area contact burn. Additional mice (n=16) received a 40{\%} burn that was totally, partially, or not excised 72 hours after burn. Excised areas were covered with normal, syngeneic skin. Two weeks later harvested splenocytes were cocultured with allogeneic stimulators. CTL activity was determined by a 51Cr release assay, in which CTL effectors were tested on allogeneic, radiolabeled targets. Dilution curves of CTL activity were compared by ANOVA. Results. Both 20{\%} and 40{\%} burns significantly inhibited CTL activity (p<0.05). Total but not partial excision of a 40{\%} burn restored CTL activity (p<0.01). Both total and partial wound excision also improved survival (p<0.05). Conclusions. Burn injury inhibits CTL activity in a size-dependent manner, and total wound excision significantly improves both CTL function and survival after injury. This study suggests a mechanism for the immunosuppressive effects of burn injury and provides an immunologic rationale for early, complete burn wound excision.",
author = "Hultman, {C. Scott} and Cairns, {Bruce A.} and Suzan deSerres and Frelinger, {Jeffrey A} and Meyer, {Anthony A.}",
year = "1995",
doi = "10.1016/S0039-6060(05)80354-X",
language = "English (US)",
volume = "118",
pages = "421--430",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Early, complete burn wound excision partially restores cytotoxic T lymphocyte function

AU - Hultman, C. Scott

AU - Cairns, Bruce A.

AU - deSerres, Suzan

AU - Frelinger, Jeffrey A

AU - Meyer, Anthony A.

PY - 1995

Y1 - 1995

N2 - Background. Cytotoxic lymphocytes (CTLs) are an important component of immune function, involved in antigen recognition and resistance to viral infection. Burn injury suppresses cell-mediated immunity, induces allograft tolerance, and increases the risk of viral infection, but the mechanisms are not well understood. This study analyzes the effect of burn size and burn wound excision on CTL activity. Methods. Anesthetized CBA mice (n=12) received a 0%, 20%, or 40% body surface area contact burn. Additional mice (n=16) received a 40% burn that was totally, partially, or not excised 72 hours after burn. Excised areas were covered with normal, syngeneic skin. Two weeks later harvested splenocytes were cocultured with allogeneic stimulators. CTL activity was determined by a 51Cr release assay, in which CTL effectors were tested on allogeneic, radiolabeled targets. Dilution curves of CTL activity were compared by ANOVA. Results. Both 20% and 40% burns significantly inhibited CTL activity (p<0.05). Total but not partial excision of a 40% burn restored CTL activity (p<0.01). Both total and partial wound excision also improved survival (p<0.05). Conclusions. Burn injury inhibits CTL activity in a size-dependent manner, and total wound excision significantly improves both CTL function and survival after injury. This study suggests a mechanism for the immunosuppressive effects of burn injury and provides an immunologic rationale for early, complete burn wound excision.

AB - Background. Cytotoxic lymphocytes (CTLs) are an important component of immune function, involved in antigen recognition and resistance to viral infection. Burn injury suppresses cell-mediated immunity, induces allograft tolerance, and increases the risk of viral infection, but the mechanisms are not well understood. This study analyzes the effect of burn size and burn wound excision on CTL activity. Methods. Anesthetized CBA mice (n=12) received a 0%, 20%, or 40% body surface area contact burn. Additional mice (n=16) received a 40% burn that was totally, partially, or not excised 72 hours after burn. Excised areas were covered with normal, syngeneic skin. Two weeks later harvested splenocytes were cocultured with allogeneic stimulators. CTL activity was determined by a 51Cr release assay, in which CTL effectors were tested on allogeneic, radiolabeled targets. Dilution curves of CTL activity were compared by ANOVA. Results. Both 20% and 40% burns significantly inhibited CTL activity (p<0.05). Total but not partial excision of a 40% burn restored CTL activity (p<0.01). Both total and partial wound excision also improved survival (p<0.05). Conclusions. Burn injury inhibits CTL activity in a size-dependent manner, and total wound excision significantly improves both CTL function and survival after injury. This study suggests a mechanism for the immunosuppressive effects of burn injury and provides an immunologic rationale for early, complete burn wound excision.

UR - http://www.scopus.com/inward/record.url?scp=0029116906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029116906&partnerID=8YFLogxK

U2 - 10.1016/S0039-6060(05)80354-X

DO - 10.1016/S0039-6060(05)80354-X

M3 - Article

VL - 118

SP - 421

EP - 430

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 2

ER -