Ecology and evolution of viruses infecting uncultivated SUP05 bacteria as revealed by single-cell- and meta-genomics

Simon Roux, Alyse K. Hawley, Monica Torres Beltran, Melanie Scofield, Patrick Schwientek, Ramunas Stepanauskas, Tanja Woyke, Steven J. Hallam, Matthew B. Sullivan

Research output: Contribution to journalArticle

Abstract

Viruses modulate microbial communities and alter ecosystem functions. However, due to cultivation bottlenecks, specific virus-host interaction dynamics remain cryptic. In this study, we examined 127 single-cell amplified genomes (SAGs) from uncultivated SUP05 bacteria isolated from a model marine oxygen minimum zone (OMZ) to identify 69 viral contigs representing five new genera within dsDNA Caudovirales and ssDNA Microviridae. Infection frequencies suggest that ∼1/3 of SUP05 bacteria is viral-infected, with higher infection frequency where oxygen-deficiency was most severe. Observed Microviridae clonality suggests recovery of bloom-terminating viruses, while systematic co-infection between dsDNA and ssDNA viruses posits previously unrecognized cooperation modes. Analyses of 186 microbial and viral metagenomes revealed that SUP05 viruses persisted for years, but remained endemic to the OMZ. Finally, identification of virus-encoded dissimilatory sulfite reductase suggests SUP05 viruses reprogram their host's energy metabolism. Together, these results demonstrate closely coupled SUP05 virus-host co-evolutionary dynamics with the potential to modulate biogeochemical cycling in climate-critical and expanding OMZs.

Original languageEnglish (US)
Pages (from-to)e03125
JournaleLife
Volume3
DOIs
StatePublished - 2014

Keywords

  • bacteriophages
  • ecology
  • infectious disease
  • microbiology
  • oxygen minimum zone
  • single cell genomics
  • SUP05
  • viral dark matter
  • viruses

ASJC Scopus subject areas

  • Medicine(all)

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    Roux, S., Hawley, A. K., Torres Beltran, M., Scofield, M., Schwientek, P., Stepanauskas, R., Woyke, T., Hallam, S. J., & Sullivan, M. B. (2014). Ecology and evolution of viruses infecting uncultivated SUP05 bacteria as revealed by single-cell- and meta-genomics. eLife, 3, e03125. https://doi.org/10.7554/eLife.03125