Edible apple film wraps containing plant antimicrobials inactivate foodborne pathogens on meat and poultry products

Sadhana Ravishankar, Libin Zhu, Carl W. Olsen, Tara H. McHugh, Mendel Friedman

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

ABSTRACT Apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. Salmonella enterica or E. coli O157:H7 (107 CFU/g) cultures were surface inoculated on chicken breasts and Listeria monocytogenes (10 6 CFU/g) on ham. The inoculated products were then wrapped with edible films containing 3 concentrations (0.5%, 1.5%, and 3%) of cinnamaldehyde or carvacrol. Following incubation at either 23 or 4 °C for 72 h, samples were stomached in buffered peptone water, diluted, and plated for enumeration of survivors. The antimicrobial films exhibited concentration-dependent activities against the pathogens tested. At 23 °C on chicken breasts, films with 3% antimicrobials showed the highest reductions (4.3 to 6.8 log CFU/g) of both S. enterica and E. coli O157:H7. Films with 1.5% and 0.5% antimicrobials showed 2.4 to 4.3 and 1.6 to 2.8 log reductions, respectively. At 4 °C, carvacrol exhibited greater activity than did cinnamaldehyde. Films with 3%, 1.5%, and 0.5% carvacrol reduced the bacterial populations by about 3, 1.6 to 3, and 0.8 to 1 logs, respectively. Films with 3% and 1.5% cinnamaldehyde induced 1.2 to 2.8 and 1.2 to 1.3 log reductions, respectively. For L. monocytogenes on ham, carvacrol films induced greater reductions than did cinnamaldehyde films at all concentrations tested. In general, the reduction of L. monocytogenes on ham at 23 °C was greater than at 4 °C. Added antimicrobials had minor effects on physical properties of the films. The results suggest that the food industry and consumers could use these films as wrappings to control surface contamination by foodborne pathogenic microorganisms.

Original languageEnglish (US)
JournalJournal of Food Science
Volume74
Issue number8
DOIs
StatePublished - Oct 2009

Fingerprint

Poultry Products
poultry products
Meat Products
Malus
food pathogens
films (materials)
meat products
Listeria monocytogenes
apples
anti-infective agents
Salmonella enterica
Escherichia coli O157
carvacrol
Chickens
Breast
ham
Peptones
Food Industry
edible films
breasts

Keywords

  • Antimicrobial edible apple films
  • Carvacrol
  • Cinnamaldehyde
  • E. coli O157:H7
  • Ham
  • Listeria monocytogenes
  • Poultry
  • Salmonella enterica

ASJC Scopus subject areas

  • Food Science

Cite this

Edible apple film wraps containing plant antimicrobials inactivate foodborne pathogens on meat and poultry products. / Ravishankar, Sadhana; Zhu, Libin; Olsen, Carl W.; McHugh, Tara H.; Friedman, Mendel.

In: Journal of Food Science, Vol. 74, No. 8, 10.2009.

Research output: Contribution to journalArticle

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abstract = "ABSTRACT Apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. Salmonella enterica or E. coli O157:H7 (107 CFU/g) cultures were surface inoculated on chicken breasts and Listeria monocytogenes (10 6 CFU/g) on ham. The inoculated products were then wrapped with edible films containing 3 concentrations (0.5{\%}, 1.5{\%}, and 3{\%}) of cinnamaldehyde or carvacrol. Following incubation at either 23 or 4 °C for 72 h, samples were stomached in buffered peptone water, diluted, and plated for enumeration of survivors. The antimicrobial films exhibited concentration-dependent activities against the pathogens tested. At 23 °C on chicken breasts, films with 3{\%} antimicrobials showed the highest reductions (4.3 to 6.8 log CFU/g) of both S. enterica and E. coli O157:H7. Films with 1.5{\%} and 0.5{\%} antimicrobials showed 2.4 to 4.3 and 1.6 to 2.8 log reductions, respectively. At 4 °C, carvacrol exhibited greater activity than did cinnamaldehyde. Films with 3{\%}, 1.5{\%}, and 0.5{\%} carvacrol reduced the bacterial populations by about 3, 1.6 to 3, and 0.8 to 1 logs, respectively. Films with 3{\%} and 1.5{\%} cinnamaldehyde induced 1.2 to 2.8 and 1.2 to 1.3 log reductions, respectively. For L. monocytogenes on ham, carvacrol films induced greater reductions than did cinnamaldehyde films at all concentrations tested. In general, the reduction of L. monocytogenes on ham at 23 °C was greater than at 4 °C. Added antimicrobials had minor effects on physical properties of the films. The results suggest that the food industry and consumers could use these films as wrappings to control surface contamination by foodborne pathogenic microorganisms.",
keywords = "Antimicrobial edible apple films, Carvacrol, Cinnamaldehyde, E. coli O157:H7, Ham, Listeria monocytogenes, Poultry, Salmonella enterica",
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AU - Friedman, Mendel

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N2 - ABSTRACT Apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. Salmonella enterica or E. coli O157:H7 (107 CFU/g) cultures were surface inoculated on chicken breasts and Listeria monocytogenes (10 6 CFU/g) on ham. The inoculated products were then wrapped with edible films containing 3 concentrations (0.5%, 1.5%, and 3%) of cinnamaldehyde or carvacrol. Following incubation at either 23 or 4 °C for 72 h, samples were stomached in buffered peptone water, diluted, and plated for enumeration of survivors. The antimicrobial films exhibited concentration-dependent activities against the pathogens tested. At 23 °C on chicken breasts, films with 3% antimicrobials showed the highest reductions (4.3 to 6.8 log CFU/g) of both S. enterica and E. coli O157:H7. Films with 1.5% and 0.5% antimicrobials showed 2.4 to 4.3 and 1.6 to 2.8 log reductions, respectively. At 4 °C, carvacrol exhibited greater activity than did cinnamaldehyde. Films with 3%, 1.5%, and 0.5% carvacrol reduced the bacterial populations by about 3, 1.6 to 3, and 0.8 to 1 logs, respectively. Films with 3% and 1.5% cinnamaldehyde induced 1.2 to 2.8 and 1.2 to 1.3 log reductions, respectively. For L. monocytogenes on ham, carvacrol films induced greater reductions than did cinnamaldehyde films at all concentrations tested. In general, the reduction of L. monocytogenes on ham at 23 °C was greater than at 4 °C. Added antimicrobials had minor effects on physical properties of the films. The results suggest that the food industry and consumers could use these films as wrappings to control surface contamination by foodborne pathogenic microorganisms.

AB - ABSTRACT Apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. Salmonella enterica or E. coli O157:H7 (107 CFU/g) cultures were surface inoculated on chicken breasts and Listeria monocytogenes (10 6 CFU/g) on ham. The inoculated products were then wrapped with edible films containing 3 concentrations (0.5%, 1.5%, and 3%) of cinnamaldehyde or carvacrol. Following incubation at either 23 or 4 °C for 72 h, samples were stomached in buffered peptone water, diluted, and plated for enumeration of survivors. The antimicrobial films exhibited concentration-dependent activities against the pathogens tested. At 23 °C on chicken breasts, films with 3% antimicrobials showed the highest reductions (4.3 to 6.8 log CFU/g) of both S. enterica and E. coli O157:H7. Films with 1.5% and 0.5% antimicrobials showed 2.4 to 4.3 and 1.6 to 2.8 log reductions, respectively. At 4 °C, carvacrol exhibited greater activity than did cinnamaldehyde. Films with 3%, 1.5%, and 0.5% carvacrol reduced the bacterial populations by about 3, 1.6 to 3, and 0.8 to 1 logs, respectively. Films with 3% and 1.5% cinnamaldehyde induced 1.2 to 2.8 and 1.2 to 1.3 log reductions, respectively. For L. monocytogenes on ham, carvacrol films induced greater reductions than did cinnamaldehyde films at all concentrations tested. In general, the reduction of L. monocytogenes on ham at 23 °C was greater than at 4 °C. Added antimicrobials had minor effects on physical properties of the films. The results suggest that the food industry and consumers could use these films as wrappings to control surface contamination by foodborne pathogenic microorganisms.

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