Effect of an arginine-glycine-aspartic acid-containing peptide on hematogenous candidal infections in rabbits

S. A. Klotz, R. L. Smith, B. W. Stewart

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

The adherence of Candida albicans yeast cells to the subendothelial extracellular matrix, fibronectin, laminin, and type I and IV collagen was tested. Fibronectin (10-7 M) and a peptide, PepTite-2000 (Telios Pharmaceuticals Inc., San Diego, Calif.), containing the sequence arginine- glycine-aspartic acid (RGD) inhibited Candida adherence to these targets by greater than 90%. When C. albicans was perfused over ex vivo rabbit aortic endothelium, there was no significant difference in the amount of adherence in the presence or absence of the RGD-containing peptide. However, the RGD- containing peptide reduced the number of Candida organisms present in liver, brain, heart, and kidneys (P < 0.05) of rabbits 4 h after intravenous inoculation of 5 x 107 C. albicans yeast cells. The peptide also reduced the number of macroscopic Candida abscesses in the kidneys of rabbits 72 h after intravenous inoculation of 107 C. albicans yeast cells (P < 0.05). Inhibition of Candida adherence in vitro and in vivo may occur because the peptide blocks a fungal receptor that is necessary for adherence.

Original languageEnglish (US)
Pages (from-to)132-136
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume36
Issue number1
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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