Effect of Calbindni-D28K on cyclosporine toxicity in cultured renal proximal tubular cells

Ming Ju Wu, Li-Wen Lai, Yeong Hau H Lien

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cyclosporine A (CsA) is known to have direct toxicity to renal tubular cells. Its toxicity may be mediated by intracellular calcium because CsA increases intracellular calcium concentration and enhances the activities of calcium-dependent calpains and caspases. Calbindin-D28k, a cytosolic calcium binding protein, has been used as an intracellular Ca2+ buffer to reduce calcium-mediated cytotoxicity in non-renal cells such as neuronal cells. We investigated the effects of gene transfer of calbindin-D 28k cDNA on CsA cytotoxicity and intracellular calcium concentration ([Ca2+]i) in cultured murine proximal tubular (MCT) cells. A plasmid containing calbindin-D28k cDNA under the control of CMV promoter was transfected to MCT cells with liposomes. Cytotoxicity was assessed by LDH release and cell viability assay, and [Ca2+]i was measured ratiometrically with fura-2. Compared with MCT cells, cells transfected with calbindin-D28k cDNA showed a reduction in LDH release by 27, 30, 32, 33, and 19% (all P < 0.05), respectively, after 24 h exposure to 1, 2.5, 5, 10, and 25 μM CsA. Cell viability after CsA treatment was also significantly higher in CB cells. A mock transfection using plasmid without calbindin-D28k cDNA insert did not affect the LDH release or cell viability after CsA treatment. CsA treatment did not affect the protein and mRNA abundance of transfected calbindin-D28k cDNA. The expression of calbindin-D28k did not affect the baseline [Ca2+] i, but significantly suppressed CsA-induced elevation in [Ca 2+]i. The expression of calbindin-D28k in renal tubular cells provides cytoprotective effects against CsA toxicity, probably through its buffering effects on [Ca2+]i.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalJournal of Cellular Physiology
Volume200
Issue number3
DOIs
StatePublished - Sep 2004

Fingerprint

Calbindin 1
Cyclosporine
Toxicity
Kidney
Complementary DNA
Calcium
Cytotoxicity
Cell Survival
Cells
Plasmids
Gene transfer
Calbindins
Calcium-Binding Proteins
Calpain
Fura-2
Caspases
Liposomes
Transfection
Assays
Buffers

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Effect of Calbindni-D28K on cyclosporine toxicity in cultured renal proximal tubular cells. / Wu, Ming Ju; Lai, Li-Wen; Lien, Yeong Hau H.

In: Journal of Cellular Physiology, Vol. 200, No. 3, 09.2004, p. 395-399.

Research output: Contribution to journalArticle

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abstract = "Cyclosporine A (CsA) is known to have direct toxicity to renal tubular cells. Its toxicity may be mediated by intracellular calcium because CsA increases intracellular calcium concentration and enhances the activities of calcium-dependent calpains and caspases. Calbindin-D28k, a cytosolic calcium binding protein, has been used as an intracellular Ca2+ buffer to reduce calcium-mediated cytotoxicity in non-renal cells such as neuronal cells. We investigated the effects of gene transfer of calbindin-D 28k cDNA on CsA cytotoxicity and intracellular calcium concentration ([Ca2+]i) in cultured murine proximal tubular (MCT) cells. A plasmid containing calbindin-D28k cDNA under the control of CMV promoter was transfected to MCT cells with liposomes. Cytotoxicity was assessed by LDH release and cell viability assay, and [Ca2+]i was measured ratiometrically with fura-2. Compared with MCT cells, cells transfected with calbindin-D28k cDNA showed a reduction in LDH release by 27, 30, 32, 33, and 19{\%} (all P < 0.05), respectively, after 24 h exposure to 1, 2.5, 5, 10, and 25 μM CsA. Cell viability after CsA treatment was also significantly higher in CB cells. A mock transfection using plasmid without calbindin-D28k cDNA insert did not affect the LDH release or cell viability after CsA treatment. CsA treatment did not affect the protein and mRNA abundance of transfected calbindin-D28k cDNA. The expression of calbindin-D28k did not affect the baseline [Ca2+] i, but significantly suppressed CsA-induced elevation in [Ca 2+]i. The expression of calbindin-D28k in renal tubular cells provides cytoprotective effects against CsA toxicity, probably through its buffering effects on [Ca2+]i.",
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