Abstract
We examined the role of estrogen in regulating the number of androgen–280 g) that were ovariectomized for 3 days were used in this study. AR numbers were determined in pituitary cytosol and nuclear fractions by binding and exchange assays using the synthetic ligand 17α-methyl-3H-trienolone. The administration of a single dose of estradiol benzoate [(EB) 10 μg/100 g BW] to ovariectomized female rats resulted in a 60% increase in cytosolic AR which was significantly (P < 0.01) elevated above that of oil-treated controls by 12 h post EB. Cytosolic AR levels remained elevated for as long as 48 h post EB (213% of controls). Saturation analysis of pituitary cytosolic AR revealed a single, high affinity binding site for 17α-methyl-3H-trienolone exhibiting an apparent dissociation constant (Kd) of 0.5 × 10-10 M in both EB- and oil-treated animals. The administration of cycloheximide (1 mg/ kg BW) before EB administration prevented the EB-induced rise in AR when measured 8 h post EB. When dihydrotestosterone (1.5 mg) was injected 24 h after EB or oil, there was a rapid increase in nuclear AR accompanied by a rapid decrease in cytosolic AR. The increase in nuclear AR was significantly greater (P < 0.05) in EB-pretreated animals vs. oil-treated controls. These observations show that a potential synergism exists between androgen and estrogen in the female rat pituitary and suggest that androgens may play an important role in regulating cyclic pituitary hormone release.
Original language | English (US) |
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Pages (from-to) | 84-89 |
Number of pages | 6 |
Journal | Endocrinology |
Volume | 121 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1987 |
Externally published | Yes |
ASJC Scopus subject areas
- Endocrinology