Effect of growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments

C. C. Schmidt, H. I. Georgescu, Chian K Kwoh, G. L. Blomstrom, C. P. Engle, L. A. Larkin, C. H. Evans, S. L Y Woo

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Growth factors have been shown to stimulate fibroblast division and thus may influence ligament healing. We analyzed the effects of individual growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments of the rabbit in vitro in order to identify growth factors that might enhance proliferation of fibroblasts and to compare the responses of the fibroblasts from the two ligaments to these growth factors. Through measurement of the uptake of [3H]-thymidine into DNA, fibroblasts from these ligaments that had been treated with epidermal growth factor and basic fibroblast growth factor were found to proliferate nearly eight times more than control fibroblasts. Additionally, the fibroblasts of both ligaments proliferated at similar rates when exposed to platelet- derived growth factor-AA, platelet-derived growth factor-BB, basic fibroblast growth factor, insulin-like growth factor-1, and interleukin-1-alpha. However, epidermal growth factor and transforming growth factor-beta caused the fibroblasts from the medial collateral ligament to proliferate at a rate 1.3-1.4 times greater than that of fibroblasts from the anterior cruciate ligament. The reverse was true with acidic fibroblast growth factor, which stimulated the fibroblasts from the anterior cruciate ligament to proliferate at a rate 1.3-1.6 times greater than that of fibroblasts from the medial collateral ligament. This study demonstrated that growth factors can stimulate cell division in ligaments and may be effective in enhancing ligament healing but that these differences were not great enough to explain fully the clinical differences observed between healing of the medial collateral and anterior cruciate ligaments.

Original languageEnglish (US)
Pages (from-to)184-190
Number of pages7
JournalJournal of Orthopaedic Research
Volume13
Issue number2
StatePublished - 1995
Externally publishedYes

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Anterior Cruciate Ligament
Intercellular Signaling Peptides and Proteins
Fibroblasts
Ligaments
Collateral Ligaments
Fibroblast Growth Factor 2
Epidermal Growth Factor
Fibroblast Growth Factor 1
Interleukin-1alpha
Platelet-Derived Growth Factor
Somatomedins
Transforming Growth Factor beta
Cell Division
Thymidine
Rabbits

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Schmidt, C. C., Georgescu, H. I., Kwoh, C. K., Blomstrom, G. L., Engle, C. P., Larkin, L. A., ... Woo, S. L. Y. (1995). Effect of growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments. Journal of Orthopaedic Research, 13(2), 184-190.

Effect of growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments. / Schmidt, C. C.; Georgescu, H. I.; Kwoh, Chian K; Blomstrom, G. L.; Engle, C. P.; Larkin, L. A.; Evans, C. H.; Woo, S. L Y.

In: Journal of Orthopaedic Research, Vol. 13, No. 2, 1995, p. 184-190.

Research output: Contribution to journalArticle

Schmidt, CC, Georgescu, HI, Kwoh, CK, Blomstrom, GL, Engle, CP, Larkin, LA, Evans, CH & Woo, SLY 1995, 'Effect of growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments', Journal of Orthopaedic Research, vol. 13, no. 2, pp. 184-190.
Schmidt, C. C. ; Georgescu, H. I. ; Kwoh, Chian K ; Blomstrom, G. L. ; Engle, C. P. ; Larkin, L. A. ; Evans, C. H. ; Woo, S. L Y. / Effect of growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments. In: Journal of Orthopaedic Research. 1995 ; Vol. 13, No. 2. pp. 184-190.
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