Effect of high electrical conductivity of hydroponic nutrient solution on vaccine protein content in transgenic tomato

Ryo Matsuda, Chieri Kubota, M. Lucrecia Alvarez, Guy A. Cardineau

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Using greenhouse tomato (Solanum lycopersicum) as a model system to produce pharmaceutical proteins, electrical conductivity (EC) of hydroponic nutrient solution was examined as a possible factor that affects the protein concentration in fruit. Transgenic tomato plants, expressing F1-V protein, a plantmade candidate subunit vaccine against plague (Yersinia pestis), were grown hydroponically at high (5.4 dS. m-1) or conventional EC [2.7 dS.m-1 (control)] with a high-wire system in a temperature-controlled greenhouse. There was no significant difference in plant growth and development including final shoot dry weight (DW), leaf area, stem elongation rate, or leaf development rate between high ECand control. Net photosynthetic rate, transpiration rate, and stomatal conductance (gS) of leaves were also not significantly different between EC treatments. For both EC treatments, immature green fruit accumulated DW at a similar rate, but dynamics observed in fruit total soluble protein (TSP) and F1-V during the fruit growth were different between the two ECs. Fruit TSP concentration per unit DW decreased while TSP content per whole fruit increased as fruit grew, regardless of EC. However, TSPs were significantly lower in high EC than in control. Fruit F1-V concentration per unitDWand F1-V content per whole fruit were also lower in high EC than in control. Our results found that increasing EC of nutrient solution decreased TSP including the vaccine protein in fruit, suggesting that adjusting nutrient solution EC at an appropriate level is necessary to avoid salinity stress in this transgenic tomato.

Original languageEnglish (US)
Pages (from-to)362-367
Number of pages6
JournalHortTechnology
Volume22
Issue number3
StatePublished - Jun 2012

Fingerprint

hydroponics
nutrient solutions
electrical conductivity
protein content
genetically modified organisms
tomatoes
vaccines
fruits
proteins
greenhouses
Yersinia pestis
biopharmaceuticals
plague
subunit vaccines
Solanum lycopersicum
leaf development
stem elongation
wire
salt stress
stomatal conductance

Keywords

  • Controlled environment agriculture
  • Molecular farming
  • Plague
  • Plant-made pharmaceuticals
  • Pmp
  • Solanum lycopersicum
  • Subunit vaccine

ASJC Scopus subject areas

  • Horticulture

Cite this

Effect of high electrical conductivity of hydroponic nutrient solution on vaccine protein content in transgenic tomato. / Matsuda, Ryo; Kubota, Chieri; Lucrecia Alvarez, M.; Cardineau, Guy A.

In: HortTechnology, Vol. 22, No. 3, 06.2012, p. 362-367.

Research output: Contribution to journalArticle

Matsuda, Ryo ; Kubota, Chieri ; Lucrecia Alvarez, M. ; Cardineau, Guy A. / Effect of high electrical conductivity of hydroponic nutrient solution on vaccine protein content in transgenic tomato. In: HortTechnology. 2012 ; Vol. 22, No. 3. pp. 362-367.
@article{4defd598083c41e097d2289fd12ce022,
title = "Effect of high electrical conductivity of hydroponic nutrient solution on vaccine protein content in transgenic tomato",
abstract = "Using greenhouse tomato (Solanum lycopersicum) as a model system to produce pharmaceutical proteins, electrical conductivity (EC) of hydroponic nutrient solution was examined as a possible factor that affects the protein concentration in fruit. Transgenic tomato plants, expressing F1-V protein, a plantmade candidate subunit vaccine against plague (Yersinia pestis), were grown hydroponically at high (5.4 dS. m-1) or conventional EC [2.7 dS.m-1 (control)] with a high-wire system in a temperature-controlled greenhouse. There was no significant difference in plant growth and development including final shoot dry weight (DW), leaf area, stem elongation rate, or leaf development rate between high ECand control. Net photosynthetic rate, transpiration rate, and stomatal conductance (gS) of leaves were also not significantly different between EC treatments. For both EC treatments, immature green fruit accumulated DW at a similar rate, but dynamics observed in fruit total soluble protein (TSP) and F1-V during the fruit growth were different between the two ECs. Fruit TSP concentration per unit DW decreased while TSP content per whole fruit increased as fruit grew, regardless of EC. However, TSPs were significantly lower in high EC than in control. Fruit F1-V concentration per unitDWand F1-V content per whole fruit were also lower in high EC than in control. Our results found that increasing EC of nutrient solution decreased TSP including the vaccine protein in fruit, suggesting that adjusting nutrient solution EC at an appropriate level is necessary to avoid salinity stress in this transgenic tomato.",
keywords = "Controlled environment agriculture, Molecular farming, Plague, Plant-made pharmaceuticals, Pmp, Solanum lycopersicum, Subunit vaccine",
author = "Ryo Matsuda and Chieri Kubota and {Lucrecia Alvarez}, M. and Cardineau, {Guy A.}",
year = "2012",
month = "6",
language = "English (US)",
volume = "22",
pages = "362--367",
journal = "HortTechnology",
issn = "1063-0198",
publisher = "American Society for Horticultural Science",
number = "3",

}

TY - JOUR

T1 - Effect of high electrical conductivity of hydroponic nutrient solution on vaccine protein content in transgenic tomato

AU - Matsuda, Ryo

AU - Kubota, Chieri

AU - Lucrecia Alvarez, M.

AU - Cardineau, Guy A.

PY - 2012/6

Y1 - 2012/6

N2 - Using greenhouse tomato (Solanum lycopersicum) as a model system to produce pharmaceutical proteins, electrical conductivity (EC) of hydroponic nutrient solution was examined as a possible factor that affects the protein concentration in fruit. Transgenic tomato plants, expressing F1-V protein, a plantmade candidate subunit vaccine against plague (Yersinia pestis), were grown hydroponically at high (5.4 dS. m-1) or conventional EC [2.7 dS.m-1 (control)] with a high-wire system in a temperature-controlled greenhouse. There was no significant difference in plant growth and development including final shoot dry weight (DW), leaf area, stem elongation rate, or leaf development rate between high ECand control. Net photosynthetic rate, transpiration rate, and stomatal conductance (gS) of leaves were also not significantly different between EC treatments. For both EC treatments, immature green fruit accumulated DW at a similar rate, but dynamics observed in fruit total soluble protein (TSP) and F1-V during the fruit growth were different between the two ECs. Fruit TSP concentration per unit DW decreased while TSP content per whole fruit increased as fruit grew, regardless of EC. However, TSPs were significantly lower in high EC than in control. Fruit F1-V concentration per unitDWand F1-V content per whole fruit were also lower in high EC than in control. Our results found that increasing EC of nutrient solution decreased TSP including the vaccine protein in fruit, suggesting that adjusting nutrient solution EC at an appropriate level is necessary to avoid salinity stress in this transgenic tomato.

AB - Using greenhouse tomato (Solanum lycopersicum) as a model system to produce pharmaceutical proteins, electrical conductivity (EC) of hydroponic nutrient solution was examined as a possible factor that affects the protein concentration in fruit. Transgenic tomato plants, expressing F1-V protein, a plantmade candidate subunit vaccine against plague (Yersinia pestis), were grown hydroponically at high (5.4 dS. m-1) or conventional EC [2.7 dS.m-1 (control)] with a high-wire system in a temperature-controlled greenhouse. There was no significant difference in plant growth and development including final shoot dry weight (DW), leaf area, stem elongation rate, or leaf development rate between high ECand control. Net photosynthetic rate, transpiration rate, and stomatal conductance (gS) of leaves were also not significantly different between EC treatments. For both EC treatments, immature green fruit accumulated DW at a similar rate, but dynamics observed in fruit total soluble protein (TSP) and F1-V during the fruit growth were different between the two ECs. Fruit TSP concentration per unit DW decreased while TSP content per whole fruit increased as fruit grew, regardless of EC. However, TSPs were significantly lower in high EC than in control. Fruit F1-V concentration per unitDWand F1-V content per whole fruit were also lower in high EC than in control. Our results found that increasing EC of nutrient solution decreased TSP including the vaccine protein in fruit, suggesting that adjusting nutrient solution EC at an appropriate level is necessary to avoid salinity stress in this transgenic tomato.

KW - Controlled environment agriculture

KW - Molecular farming

KW - Plague

KW - Plant-made pharmaceuticals

KW - Pmp

KW - Solanum lycopersicum

KW - Subunit vaccine

UR - http://www.scopus.com/inward/record.url?scp=84863214035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863214035&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 362

EP - 367

JO - HortTechnology

JF - HortTechnology

SN - 1063-0198

IS - 3

ER -