Effect of in vitro irradiation and cell cycle-inhibitory drugs on the spontaneous human IgE synthesis in vitro

Gian Franco Del Prete, Donata Vercelli, Antonio Tiri, Enrico Maggi, Oliviero Rossi, Sergio Romagnani, Mario Ricci

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The in vitro effects of radiation, diterpine forskolin (FK), and hydrocortisone (HC) on the in vitro spontaneous IgE synthesis by peripheral blood B-lymphocytes from atopic patients were investigated. Without affecting cell viability, in vitro irradiation inhibited in a dose-dependent fashion de novo IgE synthesis in vitro by B cells from all patients examined with a mean 40% reduction of in vitro IgE product, after treatment with 100 rads. In contrast, the in vitro IgE production by the U266 myeloma cell line was unaffected, even by irradiation, with 1600 rads. The addition to B cell culures from atopic patients of FK consistently resulted in a dose-dependent inhibition of the spontaneous IgE production in vitro. The addition to cultures of 10-5 and 10-6 molar concentations, of HC was also usually inhibitory, whereas lower HC concentrations were uneffective or even enhanced the spontaneous in vitro IgE synthesis. When 10-6 molar concentrations, of both HC and FK were combined in culture, a summation inhibitory effect on the spontaneous IgE synthesis was observed. In contrast, neither FK nor HC had inhibitory effect on the in vitro spontaneous IgE synthesis by the U266 myeloma cell line. The spontaneous in vitro IgE synthesis by B cells from patients with Hodgkin's disease, demonstrating high levels of serum IgE, was strongly reduced or virtually abolished after patients underwent total nodal irradiation to prevent the spread of the disease. In addition, the in vitro spontaneous IgE synthesis by B cells from atopic patients was markedly decreased or abolished by in vivo administration of betamethasone. Taken together, these data indicate that in vitro spontaneous IgE-producing cells that circulate in the peripheral blood of atopic patients or patients with Hodgkin's disease represent a heterogeneous population of B cells, most of which require progression through the proliferative cell cycle before becoming cells actively secreting IgE in vitro. (J Allergy Clin Immunol 1987:79:69-77.).

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume79
Issue number1
DOIs
StatePublished - 1987
Externally publishedYes

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Immunoglobulin E
Cell Cycle
Pharmaceutical Preparations
B-Lymphocytes
Hydrocortisone
Colforsin
Hodgkin Disease
In Vitro Techniques
Cell Line
Betamethasone
Radiation Effects
Cell Survival
Hypersensitivity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Effect of in vitro irradiation and cell cycle-inhibitory drugs on the spontaneous human IgE synthesis in vitro. / Del Prete, Gian Franco; Vercelli, Donata; Tiri, Antonio; Maggi, Enrico; Rossi, Oliviero; Romagnani, Sergio; Ricci, Mario.

In: Journal of Allergy and Clinical Immunology, Vol. 79, No. 1, 1987, p. 69-77.

Research output: Contribution to journalArticle

Del Prete, Gian Franco ; Vercelli, Donata ; Tiri, Antonio ; Maggi, Enrico ; Rossi, Oliviero ; Romagnani, Sergio ; Ricci, Mario. / Effect of in vitro irradiation and cell cycle-inhibitory drugs on the spontaneous human IgE synthesis in vitro. In: Journal of Allergy and Clinical Immunology. 1987 ; Vol. 79, No. 1. pp. 69-77.
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abstract = "The in vitro effects of radiation, diterpine forskolin (FK), and hydrocortisone (HC) on the in vitro spontaneous IgE synthesis by peripheral blood B-lymphocytes from atopic patients were investigated. Without affecting cell viability, in vitro irradiation inhibited in a dose-dependent fashion de novo IgE synthesis in vitro by B cells from all patients examined with a mean 40{\%} reduction of in vitro IgE product, after treatment with 100 rads. In contrast, the in vitro IgE production by the U266 myeloma cell line was unaffected, even by irradiation, with 1600 rads. The addition to B cell culures from atopic patients of FK consistently resulted in a dose-dependent inhibition of the spontaneous IgE production in vitro. The addition to cultures of 10-5 and 10-6 molar concentations, of HC was also usually inhibitory, whereas lower HC concentrations were uneffective or even enhanced the spontaneous in vitro IgE synthesis. When 10-6 molar concentrations, of both HC and FK were combined in culture, a summation inhibitory effect on the spontaneous IgE synthesis was observed. In contrast, neither FK nor HC had inhibitory effect on the in vitro spontaneous IgE synthesis by the U266 myeloma cell line. The spontaneous in vitro IgE synthesis by B cells from patients with Hodgkin's disease, demonstrating high levels of serum IgE, was strongly reduced or virtually abolished after patients underwent total nodal irradiation to prevent the spread of the disease. In addition, the in vitro spontaneous IgE synthesis by B cells from atopic patients was markedly decreased or abolished by in vivo administration of betamethasone. Taken together, these data indicate that in vitro spontaneous IgE-producing cells that circulate in the peripheral blood of atopic patients or patients with Hodgkin's disease represent a heterogeneous population of B cells, most of which require progression through the proliferative cell cycle before becoming cells actively secreting IgE in vitro. (J Allergy Clin Immunol 1987:79:69-77.).",
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