Effect of insulin on human skeletal muscle mitochondrial ATP production, protein synthesis, and mRNA transcripts

Craig S Stump, Kevin R. Short, Maureen L. Bigelow, Jill M. Schimke, K. Sreekumaran Nair

Research output: Contribution to journalArticle

311 Citations (Scopus)

Abstract

Mitochondria are the primary site of skeletal muscle fuel metabolism and ATP production. Although insulin is a major regulator of fuel metabolism, its effect on mitochondrial ATP production is not known. Here we report increases in vastus lateralis muscle mitochondrial ATP production capacity (32-42%) in healthy humans (P < 0.01) i.v. infused with insulin (1.5 milliunits/kg of fat-free mass per min) while clamping glucose, amino acids, glucagon, and growth hormone. Increased ATP production occurred in association with increased mRNA levels from both mitochondrial (NADH dehydrogenase subunit IV) and nuclear [cytochrome c oxidase (COX) subunit IV] genes (164-180%) encoding mitochondrial proteins (P < 0.05). In addition, muscle mitochondrial protein synthesis, and COX and citrate synthase enzyme activities were increased by insulin (P < 0.05). Further studies demonstrated no effect of low to high insulin levels on muscle mitochondrial ATP production for people with type 2 diabetes mellitus, whereas matched nondiabetic controls increased 16-26% (P (0.02) when four different substrate combinations were used. In conclusion, insulin stimulates mitochondrial oxidative phosphorylation in skeletal muscle along with synthesis of gene transcripts and mitochondrial protein in human subjects. Skeletal muscle of type 2 diabetic patients has a reduced capacity to increase ATP production with high insulin levels.

Original languageEnglish (US)
Pages (from-to)7996-8001
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number13
DOIs
StatePublished - Jun 24 2003
Externally publishedYes

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Skeletal Muscle
Adenosine Triphosphate
Insulin
Messenger RNA
Mitochondrial Proteins
Proteins
NADH Dehydrogenase
Citrate (si)-Synthase
Muscles
Glucose Clamp Technique
Muscle Proteins
Oxidative Phosphorylation
Quadriceps Muscle
Electron Transport Complex IV
Glucagon
Type 2 Diabetes Mellitus
Growth Hormone
Mitochondria
Oxidoreductases
Fats

Keywords

  • Amino acids
  • Citrate synthase
  • Cytochrome c oxidase
  • NADH dehydrogenase subunit IV

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Effect of insulin on human skeletal muscle mitochondrial ATP production, protein synthesis, and mRNA transcripts. / Stump, Craig S; Short, Kevin R.; Bigelow, Maureen L.; Schimke, Jill M.; Nair, K. Sreekumaran.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 13, 24.06.2003, p. 7996-8001.

Research output: Contribution to journalArticle

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